PDF(Pubmed)
Abstract：
In this study, we measured the growth performance and intramuscular fat (IMF) content of the Longissimus dorsi (LD) of Fuqing goats (FQs) and Nubian goats (NBYs), which exhibit extreme phenotypic differences in terms of their production and meat quality traits. RNA-Seq analysis was performed, and transcriptome data were obtained from the LD tissue of 3-month fetuses (E3), 0-month lambs (0M), 3-month lambs (3M), and 12-month lambs (12M) to reveal the differences in the molecular mechanisms regulating the muscle development and IMF deposition between FQs and NBYs. The results showed that a higher body weight and average daily gain were observed in the NBYs at three developmental stages after birth, whereas a higher IMF content was registered in the FQs at 12M. Additionally, transcriptome profiles during the embryonic period and after birth were completely different for both FQs and NBYs. Moreover, DEGs (KIF23, CCDC69, CCNA2, MKI67, KIF11, RACGAP1, NUSAP1, SKP2, ZBTB18, NES, LOC102180034, CAPN6, TUBA1A, LOC102178700, and PEG10) significantly enriched in the cell cycle (ko04110) at E3 (FQs vs. NBYs), and DEGs (MRPS7, RPS8, RPL6, RPL4, RPS11, RPS10, RPL5, RPS6, RPL8, RPS13, RPS24, RPS15, RPL23) significantly enriched in ribosomes (ko03010) at 0M (FQs vs. NBYs) related to myogenic differentiation and fusion were identified. Meanwhile, the differences in glucose and lipid metabolism began at the E3 timepoint and continued to strengthen as growth proceeded in FQs vs. NBYs. DEGs (CD36, ADIROQR2, ACACA, ACACB, CPT1A, IGF1R, IRS2, LDH-A, PKM, HK2, PFKP, PCK1, GPI, FASN, FADS1, ELOVL6, HADHB, ACOK1, ACAA2, and ACSL4) at 3M (FQs vs. NBYs) and 12M (FQs vs. NBYs) significantly enriched in the AMPK signaling pathway (ko04152), insulin resistance (ko04931), the insulin signaling pathway (ko04910), fatty acid metabolism (ko01212), and glycolysis/gluconeogenesis (ko00010) related to IMF deposition were identified. Further, the results from this study provide the basis for future studies on the mechanisms regulating muscle development and IMF deposition in different breeds of goats, and the candidate genes identified could be used in the selection process.
摘要：
在这项研究中,我们测量了福清山羊（FQs）和努比亚山羊（NBYs）的生长性能和肌肉内脂肪（IMF）含量，在生产和肉质性状方面表现出极端的表型差异。进行RNA-Seq分析，转录组数据来自3个月胎儿（E3）的LD组织，0个月的羔羊（0M），3个月的羔羊（3M），和12个月的羔羊（12M），以揭示FQs和NBYs之间调节肌肉发育和IMF沉积的分子机制的差异。结果表明，在出生后的三个发育阶段，在NBYs中观察到更高的体重和平均日增重，而在FQ中登记的IMF含量较高，为12M。此外,FQs和NBYs在胚胎期和出生后的转录组特征完全不同。此外，DEGs(KIF23,CCDC69,CCNA2,MKI67,KIF11,RACGAP1,NUSAP1,SKP2,ZBTB18,NES,LOC102180034,CAPN6,TUBA1A,LOC102178700和PEG10）在E3（FQs与NBYs),和DEG（MRPS7，RPS8，RPL6，RPL4，RPS11，RPS10，RPL5，RPS6，RPL8，RPS13，RPS24，RPS15，RPL23）在0M（FQvs.鉴定了与成肌分化和融合相关的NBYs）。同时,葡萄糖和脂质代谢的差异在E3时间点开始，并随着FQs与NBYs.DEG(CD36,ADIROQR2,ACACA,ACACB,CPT1A,IGF1R,IRS2,LDH-A,PKM,HK2,PFKP,PCK1、GPI、FASN,FADS1,ELOVL6,HADHB,ACOK1，ACAA2和ACSL4）在3M（FQ与NBYs）和12M（FQ与NBYs)显著富集在AMPK信号通路(ko04152)中，胰岛素抵抗（ko04931），胰岛素信号通路（ko04910），脂肪酸代谢（ko01212），并鉴定了与IMF沉积相关的糖酵解/糖异生(ko00010)。Further,本研究结果为今后研究不同品种山羊肌肉发育和IMF沉积的调节机制提供了依据,鉴定的候选基因可用于选择过程。
