PDF(Pubmed)
Abstract：
This study used feces from 0-day-old (36 rabbits), 10-day-old (119 rabbits), and 60-day-old (119 rabbits) offspring rabbits and their corresponding female rabbits (36 rabbits) as experimental materials. Using 16s rRNA sequencing, the study analyzed the types and changes of gut microbiota in rabbits at different growth and development stages, as well as the correlation between gut microbiota composition and the weight of 60-day-old rabbits. All experimental rabbits were placed in the same rabbit shed. Juvenile rabbits were fed solid feed at 18 days of age and weaned at 35 days of age. In addition to identifying the dominant bacterial phyla of gut microbiota in rabbits at different age stages, it was found that the abundance of Clostridium tertium and Clostridium paraputrificum in all suckling rabbits (10-day-old) was significantly higher than that in rabbits fed with whole feed (60-day-old) (p < 0.05), while the abundance of Gram-negative bacterium cTPY13 was significantly lower (p < 0.05). In addition, Fast Expected Maximum Microbial Source Tracing (FEAST) analysis showed that the contribution of female rabbits\' gut microbiota to the colonization of offspring rabbits\' gut microbiota was significantly higher than that of unrelated rabbits\' gut microbiota (p < 0.05). The contribution of female rabbits\' gut microbiota to the colonization of gut microbiota in 0-day-old rabbits was significantly higher than that to the colonization of gut microbiota in the 10- and 60-day-old rabbits (p < 0.05). Finally, the correlation between gut microbiota composition and body weight of 60-day-old rabbits was analyzed based on a mixed linear model, and six ASVs significantly affecting body weight were screened. The above results provide important theoretical and practical guidance for maintaining gut health, improving growth and development performance, and feeding formulation in rabbits.
摘要：
这项研究使用了0日龄（36只兔子）的粪便，10天大（119只兔子），和60日龄（119只）子代兔及其相应的雌性兔（36只）为实验材料。使用16srRNA测序，研究分析了不同生长发育阶段家兔肠道菌群的类型和变化,以及肠道菌群组成与60日龄兔体重的相关性。将所有实验兔置于同一兔棚内。幼年兔在18日龄时饲喂固体饲料，在35日龄时断奶。除了确定不同年龄阶段兔肠道微生物群的优势细菌门，研究发现，所有乳兔（10日龄）中的梭状芽胞杆菌和副营养梭状芽胞杆菌的丰度均显着高于全饲料（60日龄）（p<0.05），革兰阴性菌cTPY13的丰度显著降低(p<0.05)。此外,快速预期最大微生物来源追踪（FEAST）分析表明，雌性兔肠道菌群对后代兔肠道菌群定植的贡献显着高于无关兔肠道菌群（p<0.05）。雌性兔肠道菌群对0日龄兔肠道菌群定植的贡献显著高于10日龄和60日龄兔肠道菌群定植的贡献(p<0.05)。最后,基于混合线性模型分析60日龄兔肠道菌群组成与体重的相关性,筛选了6个显著影响体重的ASV。上述结果为维护肠道健康提供了重要的理论和实践指导,提高增长和发展绩效，和兔子的喂养配方。
