PDF(Pubmed)
Abstract：
Background: an accurate assessment of the immunity against SARS-CoV-2 can facilitate a better understanding and management of not only the recent coronavirus but similar pathogens as well. Objective: the aim of this study was to evaluate T-cell immunity with reference to antibody titers in a group of pediatric patients with autoimmune arthritides utilizing the widely known Interferon-γ Release Assay (IGRA). Materials and Methods: This study was conducted in the cohort of 55 children suffering from Juvenile Idiopathic Arthritis (JIA). This research analyzed the SARS-CoV-2 T-cell response measured by a specific quantitative IGRA, followed by a serological ELISA test measuring the presence and quantity of IgG, IgM, and IgA antibodies in serum. Results: The cellular response to SARS-CoV-2 measured by the IGRA test significantly correlated with the antibody titers, IgA (p < 0.00003, R = 0.537), IgG (p < 0.0001, R = 0.668), and IgG nucleocapsid protein (NCP) (p < 0.003, R = 0.0399), with no correlation with IgM levels. The antibody levels in patients receiving biological agents were significantly lower compared to the rest of the cohort (p = 0.0369), while traditional disease-modifying antirheumatic drugs had no such effect. Limitations: the main limitation of the research is the small sample size, mostly due to the specific cohort of patients and the lack of a healthy control. Conclusions: IGRA appears to be a viable tool in the accurate evaluation of T-cell responses to SARS-CoV-2, and serodiagnostics alone is not always sufficient in the assessment of immune responses.
摘要：
背景：对SARS-CoV-2的免疫力进行准确评估不仅可以促进对最近的冠状病毒以及类似病原体的更好理解和管理。目的：本研究的目的是利用广为人知的干扰素-γ释放试验（IGRA），参考一组自身免疫性关节炎的儿科患者的抗体滴度来评估T细胞免疫。材料和方法：这项研究是在55名患有幼年特发性关节炎（JIA）的儿童中进行的。这项研究分析了通过特定定量IGRA测量的SARS-CoV-2T细胞反应，随后进行血清学ELISA测试，测量IgG的存在和数量，IgM,血清中的IgA抗体.结果:IGRA检测细胞对SARS-CoV-2的反应与抗体滴度显著相关,IgA（p<0.00003，R=0.537），IgG（p<0.0001，R=0.668），和IgG核衣壳蛋白（NCP）（p<0.003，R=0.0399），与IgM水平无关。接受生物制剂的患者的抗体水平明显低于其他队列（p=0.0369），而传统的缓解疾病的抗风湿药没有这种效果。局限性：研究的主要局限性是样本量小，主要是由于特定的患者队列和缺乏健康控制。结论：IGRA似乎是准确评估T细胞对SARS-CoV-2反应的可行工具，而单独的血清诊断并不总是足以评估免疫反应。
