PDF(Pubmed)
Abstract：
Pharmaceutical and personal care products (PPCPs) containing persistent and potentially hazardous substances have garnered attention for their ubiquitous presence in natural environments. This study investigated the impact of polyethylene glycol (PEG), a common PPCP component, on Mytilus galloprovincialis. Mussels were subjected to two PEG concentrations (E1: 0.1 mg/L and E2: 10 mg/L) over 14 days. Oxidative stress markers in both gills and digestive glands were evaluated; cytotoxicity assays were performed on haemolymph and digestive gland cells. Additionally, cell volume regulation (RVD assay) was investigated to assess physiological PEG-induced alterations. In the gills, PEG reduced superoxide dismutase (SOD) activity and increased lipid peroxidation (LPO) at E1. In the digestive gland, only LPO was influenced, while SOD activity and oxidatively modified proteins (OMPs) were unaltered. A significant decrease in cell viability was observed, particularly at E2. Additionally, the RVD assay revealed disruptions in the cells subjected to E2. These findings underscore the effects of PEG exposure on M. galloprovincialis. They are open to further investigations to clarify the environmental implications of PPCPs and the possibility of exploring safer alternatives.
摘要：
含有持久性和潜在危险物质的药物和个人护理产品(PPCP)因其在自然环境中的普遍存在而受到关注。本研究调查了聚乙二醇(PEG)的影响，常见的PPCP组件，在Mytilusgalloprovincialis上。在14天内使贻贝经历两种PEG浓度(E1:0.1mg/L和E2:10mg/L)。评估了ill和消化腺中的氧化应激标志物；对血淋巴和消化腺细胞进行了细胞毒性测定。此外,研究细胞体积调节(RVD测定)以评估生理学PEG诱导的改变。在the中，PEG降低了E1处的超氧化物歧化酶（SOD）活性并增加了脂质过氧化（LPO）。在消化腺中，只有LPO受到影响，而SOD活性和氧化修饰蛋白（OMPs）没有改变。观察到细胞活力显着下降，尤其是E2。此外,RVD测定显示在接受E2的细胞中的破坏。这些发现强调了PEG暴露对galloprovincialis的影响。他们愿意进行进一步调查，以澄清PPCP对环境的影响以及探索更安全替代品的可能性。
