PDF(Pubmed)
Abstract：
The excessive production of melanin can cause skin diseases and hyperpigmentation. In this study, resveratrol contained in Dongjin rice seed (DJ526) was increased through callus induction. The antioxidant capacity of resveratrol-enriched rice callus was evaluated using the ABTS radical scavenging method and was equivalent to that of vitamin C. DJ526 rice callus extract significantly increased antioxidant activities in a concentration-dependent manner. The anti-melanogenesis effects of DJ526 rice callus extract were also evaluated in melan-a cells. Resveratrol-enriched rice callus extract significantly (i) decreased the size and number of melanin-containing cells, (ii) suppressed the activity of cellular tyrosinase and melanin content, (iii) downregulated the expression of microphthalmia-associated transcription factor, tyrosinase, tyrosinase-related protein-1, and tyrosinase-related protein-2, (iv) increased the expression of phosphorylated extracellular signal-regulated kinase 1/2 and protein kinase B, and (v) inhibited the activation of phosphorylated p38 in melan-a cells. From the above observations, DJ526 rice callus extract showed strong antioxidant and anti-melanogenesis activity at the concentration test. These findings indicate the potential of resveratrol-enriched rice callus as a novel agent for controlling hyperpigmentation.
摘要：
黑色素的过量产生可引起皮肤疾病和色素沉着过度。在这项研究中,通过愈伤组织诱导，东津水稻种子（DJ526）中的白藜芦醇含量增加。使用ABTS自由基清除方法评估了富含白藜芦醇的水稻愈伤组织的抗氧化能力，并与维生素C相当。DJ526水稻愈伤组织提取物以浓度依赖的方式显着提高了抗氧化活性。还在melan-a细胞中评估了DJ526水稻愈伤组织提取物的抗黑色素生成作用。富含白藜芦醇的水稻愈伤组织提取物显着（i）减少了含黑色素细胞的大小和数量，（ii）抑制细胞酪氨酸酶和黑色素含量的活性，（iii）下调小眼症相关转录因子的表达，酪氨酸酶,酪氨酸酶相关蛋白-1和酪氨酸酶相关蛋白-2，（iv）增加磷酸化的细胞外信号调节激酶1/2和蛋白激酶B的表达，和（v）抑制melan-a细胞中磷酸化p38的激活。从上述观察来看，DJ526水稻愈伤组织提取物在浓度试验下具有较强的抗氧化和抗黑素生成活性。这些发现表明富含白藜芦醇的水稻愈伤组织作为控制色素沉着过度的新型药剂的潜力。
