PDF(Pubmed)
Abstract：
The emerging concern about chemicals in electronic cigarettes, even those without nicotine, demands the development of advanced criteria for their exposure and risk assessment. This study aims to highlight the sensitivity of lung nuclear receptors (NRs) to electronic cigarette e-liquids, independent of nicotine presence, and the influence of the sex variable on these effects. Adult male and female C57BL/6J mice were exposed to electronic cigarettes with 0%, 3%, and 6% nicotine daily (70 mL, 3.3 s, 1 puff per min/30 min) for 14 days, using the inExpose full body chamber (SCIREQ). Following exposure, lung tissues were harvested, and RNA extracted. The expression of 84 NRs was determined using the RT2 profiler mRNA array (Qiagen). Results exhibit a high sensitivity to e-liquid exposure irrespective of the presence of nicotine, with differential expression of NRs, including one (females) and twenty-four (males) in 0% nicotine groups compared to non-exposed control mice. However, nicotine-dependent results were also significant with seven NRs (females), fifty-three NRs (males) in 3% and twenty-three NRs (female) twenty-nine NRs (male) in 6% nicotine groups, compared to 0% nicotine mice. Sex-specific changes were significant, but sex-related differences were not observed. The study provides a strong rationale for further investigation.
摘要：
对电子烟中化学物质的新关注，即使是那些没有尼古丁的人，要求为其暴露和风险评估制定先进的标准。这项研究旨在强调肺核受体（NRs）对电子烟电子液体的敏感性，独立于尼古丁的存在，以及性别变量对这些影响的影响。成年雄性和雌性C57BL/6J小鼠暴露于0％的电子烟，3%,和每天6%的尼古丁(70毫升，3.3s,1次/分钟/30分钟)持续14天，使用inExpose全身腔室(SCIREQ)。曝光后，收集肺组织，提取RNA。使用RT2分析器mRNA阵列(Qiagen)测定84个NRs的表达。结果显示，无论是否存在尼古丁，对电子液体暴露都具有很高的敏感性，随着NRs的差异表达，与未暴露的对照小鼠相比，包括0％尼古丁组中的1只（雌性）和24只（雄性）。然而,尼古丁依赖的结果也显著，有7个NRs(女性)，3％的53个NR（男性）和6％的尼古丁组中的23个NR（女性）的29个NR（男性），与0%尼古丁小鼠相比。性别特异性变化显著，但未观察到与性别相关的差异.该研究为进一步调查提供了强有力的理由。
