PDF(Pubmed)
Abstract：
The effects of exposure to airborne particulate matter with a size of 10 μm or less (PM10) on C57BL/6 mouse corneas, their response to Pseudomonas aeruginosa (PA) infection, and the protective effects of SKQ1 were determined. C57BL/6 mouse corneas receiving PBS or SKQ1 were exposed to control (air) or PM10 for 2 weeks, infected, and the disease was documented by clinical score, PMN quantitation, bacterial plate count, RT-PCR and Western blot. PBS-treated, PM10-exposed corneas did not differ at 1 day postinfection (dpi), but exhibited earlier (3 dpi) corneal thinning compared to controls. By 3 dpi, PM10 significantly increased corneal mRNA levels of several pro-inflammatory cytokines, but decreased IL-10, NQO1, GR1, GPX4, and Nrf2 over control. SKQ1 reversed these effects and Western blot selectively confirmed the RT-PCR results. PM10 resulted in higher viable bacterial plate counts at 1 and 3 dpi, but SKQ1 reduced them at 3 dpi. PM10 significantly increased MPO in the cornea at 3 dpi and was reduced by SKQ1. SKQ1, used as an adjunctive treatment to moxifloxacin, was not significantly different from moxifloxacin alone. Exposure to PM10 increased the susceptibility of C57BL/6 to PA infection; SKQ1 significantly reversed these effects, but was not effective as an adjunctive treatment.
摘要：
暴露于10μm或更小的空气颗粒物（PM10）对C57BL/6小鼠角膜的影响，他们对铜绿假单胞菌（PA）感染的反应，并确定SKQ1的保护作用。接受PBS或SKQ1的C57BL/6小鼠角膜暴露于对照（空气）或PM102周，感染,通过临床评分记录疾病，PMN定量，细菌平板计数，RT-PCR和Westernblot。PBS处理，暴露于PM10的角膜在感染后1天（dpi）没有差异，但与对照组相比，角膜变薄更早（3dpi）。通过3dpi，PM10显著增加几种促炎细胞因子的角膜mRNA水平，但IL-10，NQO1，GR1，GPX4和Nrf2比对照组降低。SKQ1逆转了这些作用,并且Western印迹选择性地证实了RT-PCR结果。PM10在1和3dpi时导致更高的活细菌平板计数，但SKQ1在3dpi时降低了它们。PM10在3dpi时显着增加角膜中的MPO，并通过SKQ1减少。SKQ1，用作莫西沙星的辅助治疗，与单独的莫西沙星没有显着差异。暴露于PM10增加了C57BL/6对PA感染的易感性;SKQ1显著逆转了这些效应,但作为辅助治疗无效。
