PDF(Pubmed)
Abstract：
Probiotics have become increasingly recognized for their potential health-promoting properties; however, the viability of probiotics can be affected by storage and transportation processes as well as the stressful environment of the human digestive tract, preventing them from achieving effective concentration (107 CFU/mL). In this regard, the embedding technology of probiotics provides an effective protection method. Dextran-based water in water (W/W) emulsion loaded with Lactobacillus plantarum was used as spinning solution to prepare Lactobacillus plantarum-loaded electrospun fibers. The structure of the W/W emulsion and the electrospun fibers was charactered. Lactobacillus plantarum were uniformly embedded in the internal phase of the W/W emulsion and the loading efficiency was 9.70 ± 0.40 log CFU/g. After 240 min digestion in the gastrointestinal tract, and temperature treatment in 65 °C and 72 °C, the loaded probiotics maintained high activity. Even after 5 days of storage in room temperature and 4 °C, the loaded probiotic activity levels remained high, with counts >8 log CFU/g. These results suggest that probiotics encapsulated by emulsion electrospinning could be potentially delivered in a novel food delivery system used in the future food industry.
摘要：
益生菌因其潜在的健康促进特性而日益受到认可；然而，益生菌的生存能力会受到储存和运输过程以及人体消化道压力环境的影响，防止它们达到有效浓度（107CFU/mL）。在这方面,益生菌的包埋技术提供了一种有效的保护方法。将负载有植物乳杆菌的基于葡聚糖的水包水(W/W)乳液用作纺丝溶液以制备负载植物乳杆菌的电纺纤维。表征了W/W乳液和电纺纤维的结构。将植物乳杆菌均匀包埋在W/W乳液的内相中,装载效率为9.70±0.40logCFU/g。在胃肠道消化240分钟后，和65°C和72°C的温度处理，负载的益生菌保持高活性。即使在室温和4°C下储存5天后，加载的益生菌活性水平仍然很高，计数>8logCFU/g。这些结果表明，通过乳液静电纺丝包封的益生菌可以潜在地在未来食品工业中使用的新型食品递送系统中递送。
