PDF(Pubmed)
Abstract：
Hydroalcoholic extracts from Malbec and Torrontés wine pomaces (Vitis vinifera L.) originating from the high-altitude vineyards of Argentina\'s Calchaquí Valleys were characterized. Total phenolics, hydroxycinnamic acids, orthodiphenols, anthocyanins, non-flavonoid phenolics, total flavonoids, flavones/flavonols, flavanones/dihydroflavonols, and tannins were quantified through spectrophotometric methods, with the Malbec extract exhibiting higher concentrations in most of phytochemical groups when compared to Torrontés. HPLC-DAD identified more than 30 phenolic compounds in both extracts. Malbec displayed superior antiradical activity (ABTS cation, nitric oxide, and superoxide anion radicals), reduction power (iron, copper, and phosphomolybdenum), hypochlorite scavenging, and iron chelating ability compared to Torrontés. The cytotoxicity assessments revealed that Torrontés affected the viability of HT29-MTX and Caco-2 colon cancer cells by 70% and 50%, respectively, at the highest tested concentration (1 mg/mL). At the same time, both extracts did not demonstrate acute toxicity in Artemia salina or in red blood cell assays at 500 µg/mL. Both extracts inhibited the lipoxygenase enzyme (IC50: 154.7 and 784.7 µg/mL for Malbec and Torrontés), with Malbec also reducing the tyrosinase activity (IC50: 89.9 µg/mL), and neither inhibited the xanthine oxidase. The substantial phenolic content and diverse biological activities in the Calchaquí Valleys\' pomaces underline their potentialities to be valorized for pharmaceutical, cosmetic, and food industries.
摘要：
表征了来自阿根廷Calchaquí山谷高海拔葡萄园的Malbec和Torrontés酒窖（VitisviniferaL.）的水醇提取物。总酚类物质,羟基肉桂酸，邻二酚，花青素,非类黄酮酚类物质,总黄酮,黄酮/黄酮醇，黄烷酮/二氢黄酮醇，单宁通过分光光度法定量，与Torrontés相比，Malbec提取物在大多数植物化学组中表现出更高的浓度。HPLC-DAD在两种提取物中鉴定出30多种酚类化合物。马尔贝克表现出优异的抗自由基活性(ABTS阳离子，一氧化氮,和超氧阴离子自由基)，还原功率(铁，铜，和磷钼)，清除次氯酸盐，和铁螯合能力相比Torrontés.细胞毒性评估显示，Torrontés影响HT29-MTX和Caco-2结肠癌细胞的活力达70％和50％，分别,在最高测试浓度（1毫克/毫升）。同时,两种提取物在500µg/mL的卤虫或红细胞试验中均未显示急性毒性.两种提取物都抑制了脂氧合酶（Malbec和Torrontés的IC50：154.7和784.7µg/mL），Malbec也降低了酪氨酸酶活性（IC50：89.9µg/mL），也不抑制黄嘌呤氧化酶.CalchaquíValleys\'pomaces中大量的酚类含量和多种生物活性突显了它们在制药方面的潜力，化妆品,和食品工业。
