PDF(Pubmed)
Abstract：
Bacterial contamination is a hazard in many industries, including food, pharmaceuticals, and healthcare. The availability of a rapid and simple method for detecting this type of contamination in sterile areas enables immediate intervention to avoid or reduce detrimental effects. Among these methods, colorimetric indicators are becoming increasingly popular due to their affordability, ease of use, and quick visual interpretation of the signal. In this article, a bacterial contamination indicator system was designed by incorporating MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) into an electrospun PADAS matrix, which is a biodegradable poly(ester amide) synthesized from L-alanine, 1,12-dodecanediol, and sebacic acid. Uniaxial stress testing, thermogravimetric analysis and scanning electron microscopy were used to examine the mechanical properties, thermal stability, and morphology of the mats, respectively. The capacity for bacterial detection was not only analyzed with agar and broth assays but also by replicating important environmental conditions. Among the MTT concentrations tested in this study (0.2%, 2%, and 5%), it was found that only with a 2% MTT content the designed system produced a color response visible to the naked eye with optimal intensity, a sensitivity limit of 104 CFU/mL, and 86% cell viability, which showed the great potential for its use to detect bacterial contamination. In summary, by means of the process described in this work, it was possible to obtain a simple, low-cost and fast-response bacterial contamination indicator that can be used in mask filters, air filters, or protective clothing.
摘要：
细菌污染在许多行业都是一种危害，包括食物,制药，和医疗保健。用于在无菌区域中检测这种类型的污染的快速且简单的方法的可用性使得能够立即干预以避免或减少有害影响。在这些方法中,比色指示剂由于其可负担性而变得越来越受欢迎，易用性,和信号的快速视觉解释。在这篇文章中,通过将MTT（3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴化物）掺入到电纺PADAS基质中来设计细菌污染指示系统，它是由L-丙氨酸合成的可生物降解的聚（酯酰胺），1,12-十二烷二醇,还有癸二酸.单轴应力测试,使用热重分析和扫描电子显微镜检查机械性能，热稳定性，和垫子的形态，分别。不仅通过琼脂和肉汤测定法分析了细菌检测的能力，还通过复制重要的环境条件进行了分析。在本研究测试的MTT浓度中(0.2%，2%,和5%)，结果发现，只有2％的MTT含量的设计系统产生的颜色反应肉眼可见的最佳强度，灵敏度极限为104CFU/mL，和86%的细胞活力，这显示了其用于检测细菌污染的巨大潜力。总之,通过这项工作中描述的过程，有可能获得一个简单的，低成本和快速响应的细菌污染指示器，可用于面罩过滤器，空气过滤器,或防护服。
