PDF(Pubmed)
Abstract：
We fabricated a microfluidic chip (osteoblast [OB]-osteoclast [OC] chip) that could regulate the mixture amounts of OB and OC supernatants to investigate the effect of different supernatant distributions on osteogenesis or osteoclastogenesis. Computer-aided design was used to produce an OB-OC chip from polydimethylsiloxane. A pressure controller was assembled and different blends of OB and OC supernatants were correctly determined. OB and OC supernatants were placed on the upper panels of the OB-OC chip after differentiation for an in vitro evaluation. We then tested the changes in osteogenesis using MC3T3-E1 cells in the middle chambers. We observed that a 75:25 distribution of OB and OC supernatants was the most potent in osteogenesis. We then primed the osteogenic differentiation of MC3T3-E1 cells using an OB-OC mixed supernatant or an OB supernatant alone (supernatant ratios of 75:25 or 100:0, respectively). These cells were placed on the calvarial defect sites of rats. Microcomputed tomography and histological analyses determined a significantly higher bone formation in the group exposed to the OB-OC supernatant at a ratio of 75:25. In this study, we demonstrate the applicability of an OB-OC chip to evaluate the effect of different supernatant distributions of OB and OC. We observed that the highest bone-forming potential was in MC3T3-E1 cells treated with conditioned media, specifically the OB-OC supernatant at a ratio of 75:25.
摘要：
我们制作了一种微流控芯片（成骨细胞[OB]-破骨细胞[OC]芯片），可以调节OB和OC上清液的混合量，以研究不同上清液分布对成骨或破骨细胞生成的影响。计算机辅助设计用于由聚二甲基硅氧烷生产OB-OC芯片。组装压力控制器并正确地确定OB和OC上清液的不同混合物。分化后,将OB和OC上清液置于OB-OC芯片的上面板上,用于体外评估。然后，我们使用中室中的MC3T3-E1细胞测试了成骨的变化。我们观察到OB和OC上清液的75:25分布在成骨中最有效。然后,我们使用OB-OC混合上清液或单独的OB上清液(上清液比例分别为75:25或100:0)引发MC3T3-E1细胞的成骨分化。将这些细胞置于大鼠的颅骨缺损部位。显微计算机断层扫描和组织学分析确定以75:25的比例暴露于OB-OC上清液的组中的骨形成明显更高。在这项研究中,我们证明了OB-OC芯片评估OB和OC不同上清液分布的影响的适用性。我们观察到，最高的骨形成潜力是在用条件培养基处理的MC3T3-E1细胞中，具体地,OB-OC上清液的比例为75:25。
