PDF(Pubmed)
Abstract：
Aging (senescence) is an unavoidable biological process that results in visible manifestations in all cutaneous tissues, including scalp skin and hair follicles. Previously, we evaluated the molecular function of adenosine in promoting alopecia treatment in vitro. To elucidate the differences in the molecular mechanisms between minoxidil (MNX) and adenosine, gene expression changes in dermal papilla cells were examined. The androgen receptor (AR) pathway was identified as a candidate target of adenosine for hair growth, and the anti-androgenic activity of adenosine was examined in vitro. In addition, ex vivo examination of human hair follicle organ cultures revealed that adenosine potently elongated the anagen stage. According to the severity of alopecia, the ratio of the two peaks (terminal hair area/vellus hair area) decreased continuously. We further investigated the adenosine hair growth promoting effect in vivo to examine the hair thickness growth effects of topical 5% MNX and the adenosine complex (0.75% adenosine, 1% penthenol, and 2% niacinamide; APN) in vivo. After 4 months of administration, both the MNX and APN group showed significant increases in hair density (MNX + 5.01% (p < 0.01), APN + 6.20% (p < 0.001)) and thickness (MNX + 5.14% (p < 0.001), APN + 10.32% (p < 0.001)). The inhibition of AR signaling via adenosine could have contributed to hair thickness growth. We suggest that the anti-androgenic effect of adenosine, along with the evaluation of hair thickness distribution, could help us to understand hair physiology and to investigate new approaches for drug development.
摘要：
衰老（衰老）是一个不可避免的生物学过程，导致在所有皮肤组织中可见的表现，包括头皮皮肤和毛囊。以前,我们评估了腺苷在体外促进脱发治疗中的分子功能。为了阐明米诺地尔（MNX）和腺苷之间的分子机制的差异，检查了真皮乳头细胞的基因表达变化。雄激素受体（AR）途径被确定为用于毛发生长的腺苷的候选靶标，体外研究了腺苷的抗雄激素活性。此外,人毛囊器官培养物的离体检查显示，腺苷可有效延长生长期。根据脱发的严重程度,两个峰的比率(终毛面积/毫毛面积)连续下降。我们进一步研究了腺苷在体内的毛发生长促进作用，以检查局部5％MNX和腺苷复合物（0.75％腺苷，1%对等酚,和2%烟酰胺；APN）体内。给药4个月后，MNX和APN组头发密度均显著增加(MNX+5.01%(p<0.01),APN+6.20%(p<0.001))和厚度(MNX+5.14%(p<0.001)，APN+10.32%(p<0.001))。通过腺苷抑制AR信号可能有助于毛发厚度的生长。我们建议腺苷的抗雄激素作用，随着头发厚度分布的评估，可以帮助我们了解头发生理学并研究药物开发的新方法。
