PDF(Pubmed)
Abstract：
Complex gut microbiota increases chickens\' resistance to enteric pathogens. However, the principles of this phenomenon are not understood in detail. One of the possibilities for how to decipher the role of gut microbiota in chickens\' resistance to enteric pathogens is to systematically characterise the gene expression of individual gut microbiota members colonising the chicken caecum. To reach this aim, newly hatched chicks were inoculated with bacterial species whose whole genomic sequence was known. Total protein purified from the chicken caecum was analysed by mass spectrometry, and the obtained spectra were searched against strain-specific protein databases generated from known genomic sequences. Campylobacter jejuni, Phascolarctobacterium sp. and Sutterella massiliensis did not utilise carbohydrates when colonising the chicken caecum. On the other hand, Bacteroides, Mediterranea, Marseilla, Megamonas, Megasphaera, Bifidobacterium, Blautia, Escherichia coli and Succinatimonas fermented carbohydrates. C. jejuni was the only motile bacterium, and Bacteroides mediterraneensis expressed the type VI secretion system. Classification of in vivo expression is key for understanding the role of individual species in complex microbial populations colonising the intestinal tract. Knowledge of the expression of motility, the type VI secretion system, and preference for carbohydrate or amino acid fermentation is important for the selection of bacteria for defined competitive exclusion products.
摘要：
复杂的肠道菌群增加了鸡对肠道病原体的抵抗力。然而,这种现象的原理还没有得到详细的理解。如何破译肠道微生物群在鸡对肠道病原体的抗性中的作用的可能性之一是系统地表征在鸡盲肠中定居的单个肠道微生物群成员的基因表达。为了达到这个目的,用已知整个基因组序列的细菌物种接种新孵化的小鸡。通过质谱分析从鸡盲肠中纯化的总蛋白，并针对从已知基因组序列产生的菌株特异性蛋白质数据库搜索获得的光谱。空肠弯曲杆菌,相颈杆菌属。和Sutterellamassiliensis在定居鸡盲肠时没有利用碳水化合物。另一方面,拟杆菌,地中海,马赛拉,Megamonas,Megasphaera,双歧杆菌,Blautia,大肠杆菌和琥珀酸发酵碳水化合物。空肠弯曲杆菌是唯一的能动细菌，和地中海拟杆菌表达VI型分泌系统。体内表达的分类对于理解单个物种在肠道定殖的复杂微生物群体中的作用至关重要。了解运动的表达，VI型分泌系统，对碳水化合物或氨基酸发酵的偏好对于选择确定的竞争性排斥产物的细菌很重要。
