PDF(Pubmed)
Abstract：
Ruthenium chloride (RuCl3) is widely utilized for synthesis and catalysis of numerous compounds in academia and industry and is utilized as a key molecule in a variety of compounds with medical applications. Interestingly, RuCl3 has been demonstrated to modulate human plasmatic coagulation and serves as a constituent of a compounded inorganic antivenom that neutralizes the coagulopathic effects of snake venom in vitro and in vivo. Using thrombelastography, this investigation sought to determine if RuCl3 inhibition of the fibrinogenolytic effects of Crotalus atrox venom could be modulated by vehicle composition in human plasma. Venom was exposed to RuCl3 in 0.9% NaCl, phosphate-buffered saline (PBS), or 0.9% NaCl containing 1% dimethyl sulfoxide (DMSO). RuCl3 inhibited venom-mediated delay in the onset of thrombus formation, decreased clot growth velocity, and decreased clot strength. PBS and DMSO enhanced the effects of RuCl3. It is concluded that while a Ru-based cation is responsible for significant inhibition of venom activity, a combination of Ru-based ions containing phosphate and DMSO enhances RuCl3-mediated venom inhibition. Additional investigation is indicated to determine what specific Ru-containing molecules cause venom inhibition and what other combinations of inorganic/organic compounds may enhance the antivenom effects of RuCl3.
摘要：
氯化钌(RuCl3)在学术界和工业界广泛用于许多化合物的合成和催化,并且在具有医学应用的各种化合物中被用作关键分子。有趣的是,已证明RuCl3可调节人的血浆凝血，并可作为复合无机抗蛇毒血清的组成部分，在体内和体外中和蛇毒的凝血效应。使用血栓弹力图,这项研究试图确定RuCl3对Crotalusatrox毒液纤溶作用的抑制作用是否可以通过人血浆中的载体成分来调节。毒液在0.9%NaCl中暴露于RuCl3,磷酸盐缓冲盐水（PBS），或含1%二甲基亚砜(DMSO)的0.9%NaCl。RuCl3抑制了毒液介导的血栓形成延迟，凝块生长速度降低，凝块强度降低。PBS和DMSO增强了RuCl3的作用。结论是，虽然Ru基阳离子对毒液活性有显著的抑制作用，含有磷酸根和DMSO的Ru基离子的组合增强RuCl3介导的毒液抑制。指出了进一步的研究，以确定哪些特定的含Ru分子会引起毒液抑制，以及哪些其他无机/有机化合物的组合可能会增强RuCl3的抗蛇毒作用。
