PDF(Pubmed)
Abstract：
The maturation of HIV-1 virions is a crucial process in viral replication. Although T-cells are a primary source of virus production, much of our understanding of virion maturation comes from studies using the HEK293T human embryonic kidney cell line. Notably, there is a lack of comparative analyses between T-cells and HEK293T cells in terms of virion maturation efficiency in existing literature. We previously developed an advanced virion visualization system based on the FRET principle, enabling the effective distinction between immature and mature virions via fluorescence microscopy. In this study, we utilized pseudotyped, single-round infectious viruses tagged with FRET labels (HIV-1 Gag-iFRET∆Env) derived from Jurkat (a human T-lymphocyte cell line) and HEK293T cells to evaluate their virion maturation rates. HEK293T-derived virions demonstrated a maturity rate of 81.79%, consistent with other studies and our previous findings. However, virions originating from Jurkat cells demonstrated a significantly reduced maturation rate of 68.67% (p < 0.0001). Correspondingly, viruses produced from Jurkat cells exhibited significantly reduced infectivity compared to those derived from HEK293T cells, with the relative infectivity measured at 65.3%. This finding is consistent with the observed relative maturation rate of viruses produced by Jurkat cells. These findings suggest that initiation of virion maturation directly correlates with viral infectivity. Our observation highlights the dynamic nature of virus-host interactions and their implications for virion production and infectivity.
摘要：
HIV-1病毒粒子的成熟是病毒复制的关键过程。虽然T细胞是病毒生产的主要来源，我们对病毒体成熟的大部分理解来自使用HEK293T人胚肾细胞系的研究。值得注意的是,现有文献中缺乏T细胞和HEK293T细胞在病毒体成熟效率方面的比较分析.我们之前基于FRET原理开发了一种先进的病毒体可视化系统，通过荧光显微镜有效区分未成熟和成熟的病毒体。在这项研究中,我们利用假型，用来自Jurkat（人T淋巴细胞细胞系）和HEK293T细胞的FRET标记（HIV-1Gag-iFRETΔEnv）标记的单轮感染性病毒，以评估其病毒体成熟率。HEK293T衍生的病毒体的成熟率为81.79%，与其他研究和我们之前的发现一致。然而,源自Jurkat细胞的病毒粒子显示出显著降低的68.67%的成熟率(p<0.0001)。相应地,与来自HEK293T细胞的病毒相比，由Jurkat细胞产生的病毒显示出显着降低的感染性，相对感染性测量为65.3%。这一发现与观察到的由Jurkat细胞产生的病毒的相对成熟率一致。这些发现表明，病毒体成熟的启动与病毒感染性直接相关。我们的观察强调了病毒与宿主相互作用的动态性质及其对病毒体产生和传染性的影响。
