PDF(Pubmed)
Abstract：
The GRAS gene family, responsible for encoding transcription factors, serves pivotal functions in plant development, growth, and responses to stress. The exploration of the GRAS gene family within the Orchidaceae has been comparatively limited, despite its identification and functional description in various plant species. This study aimed to conduct a thorough examination of the GRAS gene family in Cymbidum goeringii, focusing on its physicochemical attributes, phylogenetic associations, gene structure, cis-acting elements, and expression profiles under heat stress. The results show that a total of 54 CgGRASs were pinpointed from the genome repository and categorized into ten subfamilies via phylogenetic associations. Assessment of gene sequence and structure disclosed the prevalent existence of the VHIID domain in most CgGRASs, with around 57.41% (31/54) CgGRASs lacking introns. The Ka/Ks ratios of all CgGRASs were below one, indicating purifying selection across all CgGRASs. Examination of cis-acting elements unveiled the presence of numerous elements linked to light response, plant hormone signaling, and stress responsiveness. Furthermore, CgGRAS5 contained the highest quantity of cis-acting elements linked to stress response. Experimental results from RT-qPCR demonstrated notable variations in the expression levels of eight CgGRASs after heat stress conditions, particularly within the LAS, HAM, and SCL4/7 subfamilies. In conclusion, this study revealed the expression pattern of CgGRASs under heat stress, providing reference for further exploration into the roles of CgGRAS transcription factors in stress adaptation.
摘要：
GRAS基因家族,负责编码转录因子，在植物发育中起关键作用，增长,和对压力的反应。兰科的GRAS基因家族的探索相对有限，尽管它在各种植物物种中得到了鉴定和功能描述。本研究的目的是对春藤的GRAS基因家族进行彻底的检查，专注于它的物理化学属性，系统发育关联，基因结构，顺式作用元素，和热应激下的表达谱。结果表明，从基因组库中确定了总共54个CgGRASs，并通过系统发育关联分为10个亚家族。对基因序列和结构的评估揭示了VHIID结构域在大多数CgGRASs中的普遍存在，约57.41%(31/54)的CgGRASs缺乏内含子。所有CgGRASs的Ka/Ks比值均低于1，指示所有CgGRASs的纯化选择。对顺式作用元素的检查揭示了与光响应相关的许多元素的存在，植物激素信号，和压力反应。此外，CgGRAS5含有与应激反应相关的最大量的顺式作用元件。RT-qPCR的实验结果表明，在热应激条件下，八个CgGRASs的表达水平发生了显着变化，特别是在LAS内部，HAM,和SCL4/7亚家族。总之,这项研究揭示了CgGRASs在热应激下的表达模式，为进一步探讨CgGRAS转录因子在应激适应中的作用提供参考。
