PDF(Pubmed)
Abstract：
Mesenchymal adipose stromal cells (ASCs) are considered the most promising and accessible material for translational medicine. ASCs can be used independently or within the structure of scaffold-based constructs, as these not only ensure mechanical support, but can also optimize conditions for cell activity, as specific features of the scaffold structure have an impact on the vital activity of the cells. This manuscript presents a study of the secretion and accumulation that occur in a conditioned medium during the cultivation of human ASCs within the structure of such a partial skin-equivalent that is in contact with it. It is demonstrated that the ASCs retain their functional activity during cultivation both within this partial skin-equivalent structure and, separately, on plastic substrates: they proliferate and secrete various proteins that can then accumulate in the conditioned media. Our comparative study of changes in the conditioned media during cultivation of ASCs on plastic and within the partial skin-equivalent structure reveals the different dynamics of the release and accumulation of such secretory factors in the media under a variety of conditions of cell functioning. It is also demonstrated that the optimal markers for assessment of the ASCs\' secretory functions in the studied partial skin-equivalent structure are the trophic factors VEGF-A, HGF, MCP, SDF-1α, IL-6 and IL-8. The results will help with the development of an algorithm for preclinical studies of this skin-equivalent in vitro and may be useful in studying various other complex constructs that include ASCs.
摘要：
间充质脂肪基质细胞（ASCs）被认为是转化医学最有希望和最容易获得的材料。ASC可以独立使用或在基于支架的构建体的结构内使用，因为这些不仅确保机械支撑，但也可以优化细胞活动的条件，因为支架结构的特定特征对细胞的重要活动有影响。该手稿提出了对在与之接触的这种部分皮肤等效物的结构中培养人ASC期间在条件培养基中发生的分泌和积累的研究。已证明，ASC在该部分皮肤等效结构内的培养过程中保留了其功能活性，分开,在塑料基质上：它们增殖并分泌各种蛋白质，然后可以在条件培养基中积累。我们对ASC在塑料上和部分皮肤等效结构内培养过程中条件培养基变化的比较研究揭示了在各种细胞功能条件下，此类分泌因子在培养基中释放和积累的不同动力学。还证明了在所研究的部分皮肤等效结构中评估ASC分泌功能的最佳标记是营养因子VEGF-A，HGF,MCP,SDF-1α,IL-6和IL-8。结果将有助于开发用于这种皮肤等效体外临床前研究的算法，并且可能有助于研究包括ASC在内的各种其他复杂构建体。
