PDF(Pubmed)
Abstract：
BACKGROUND: Intraoperative frozen sections (FS) are frequently used to establish the diagnosis of lung cancer when preoperative examinations are not conclusive. The downside of FS is its resource-intensive nature and the risk of tissue depletion when small lesions are assessed. Ex vivo fluorescence confocal microscopy (FCM) is a novel microimaging method for loss-free examinations of native materials. We tested its suitability for the intraoperative diagnosis of lung tumors.
METHODS: Samples from 59 lung resection specimens containing 45 carcinomas were examined in the FCM. The diagnostic performance in the evaluation of malignancy and histological typing of lung tumors was evaluated in comparison with FS and the final diagnosis.
RESULTS: A total of 44/45 (98%) carcinomas were correctly identified as malignant in the FCM. A total of 33/44 (75%) carcinomas were correctly subtyped, which was comparable with the results of FS and conventional histology. Our tests documented the excellent visualization of cytological features of normal tissues and tumors. Compared to FS, FCM was technically less demanding and less personnel intensive.
CONCLUSIONS: The ex vivo FCM is a fast, effective, and safe method for diagnosing and subtyping lung cancer and is, therefore, a promising alternative to FS. The method preserves the tissue without loss for subsequent examinations, which is an advantage in the diagnosis of small tumors and for biobanking.
METHODS: Samples from 59 lung resection specimens containing 45 carcinomas were examined in the FCM. The diagnostic performance in the evaluation of malignancy and histological typing of lung tumors was evaluated in comparison with FS and the final diagnosis.
RESULTS: A total of 44/45 (98%) carcinomas were correctly identified as malignant in the FCM. A total of 33/44 (75%) carcinomas were correctly subtyped, which was comparable with the results of FS and conventional histology. Our tests documented the excellent visualization of cytological features of normal tissues and tumors. Compared to FS, FCM was technically less demanding and less personnel intensive.
CONCLUSIONS: The ex vivo FCM is a fast, effective, and safe method for diagnosing and subtyping lung cancer and is, therefore, a promising alternative to FS. The method preserves the tissue without loss for subsequent examinations, which is an advantage in the diagnosis of small tumors and for biobanking.
摘要：
背景：术中冰冻切片（FS）通常用于在术前检查尚无定论时确定肺癌的诊断。FS的缺点是其资源密集型性质和评估小病变时组织耗竭的风险。离体荧光共聚焦显微镜（FCM）是一种新颖的显微成像方法，用于对天然材料进行无损检查。我们测试了其对肺肿瘤术中诊断的适用性。
方法：在FCM中检查了59个包含45个癌的肺切除标本的样本。与FS和最终诊断相比，评估了肺部肿瘤的恶性评估和组织学分型的诊断性能。
结果：在FCM中，共有44/45（98%）的癌被正确识别为恶性。共有33/44（75％）的癌被正确分型，与FS和常规组织学结果相当。我们的测试记录了正常组织和肿瘤的细胞学特征的出色可视化。与FS相比，FCM在技术上要求较低，人员密集程度较低。
结论：离体FCM是一种快速，有效,和诊断和分型肺癌的安全方法，因此,一个有希望的替代FS。该方法保留了组织而没有损失，用于随后的检查，这在诊断小肿瘤和生物分析中是一个优势。
方法：在FCM中检查了59个包含45个癌的肺切除标本的样本。与FS和最终诊断相比，评估了肺部肿瘤的恶性评估和组织学分型的诊断性能。
结果：在FCM中，共有44/45（98%）的癌被正确识别为恶性。共有33/44（75％）的癌被正确分型，与FS和常规组织学结果相当。我们的测试记录了正常组织和肿瘤的细胞学特征的出色可视化。与FS相比，FCM在技术上要求较低，人员密集程度较低。
结论：离体FCM是一种快速，有效,和诊断和分型肺癌的安全方法，因此,一个有希望的替代FS。该方法保留了组织而没有损失，用于随后的检查，这在诊断小肿瘤和生物分析中是一个优势。
