PDF(Pubmed)
Abstract：
Autologous-engineered artificial tissues constitute an ideal alternative for radical surgery in terms of natural anticoagulation, self-repair, tissue regeneration, and the possibility of growth. Previously, we focused on the development and practical application of artificial tissues using \"in-body tissue architecture (iBTA)\", a technique that uses living bodies as bioreactors. This study aimed to further develop iBTA by fabricating tissues with diverse shapes and evaluating their physical properties. Although the breaking strength increased with tissue thickness, the nominal breaking stress increased with thinner tissues. By carving narrow grooves on the outer periphery of an inner core with narrow grooves, we fabricated approximately 2.2 m long cord-shaped tissues and net-shaped tissues with various designs. By assembling the two inner cores inside the branched stainless-steel pipes, a large graft with branching was successfully fabricated, and its aortic arch replacement was conducted in a donor goat without causing damage. In conclusion, by applying iBTA technology, we have made it possible, for the first time, to create tissues of various shapes and designs that are difficult using existing tissue-engineering techniques. Thicker iBTA-induced tissues exhibited higher rupture strength; however, rupture stress was inversely proportional to thickness. These findings broaden the range of iBTA-induced tissue applications.
摘要：
自体工程人工组织在自然抗凝方面是根治性手术的理想选择，自我修复,组织再生，增长的可能性。以前,我们专注于使用“体内组织架构(iBTA)”的人造组织的开发和实际应用，一种使用活体作为生物反应器的技术。这项研究旨在通过制造具有不同形状的组织并评估其物理性质来进一步开发iBTA。虽然断裂强度随组织厚度的增加而增加,名义断裂应力随组织变薄而增加。通过在具有窄凹槽的内芯的外周上雕刻窄凹槽，我们制作了大约2.2m长的绳状组织和各种设计的网状组织。通过在分支的不锈钢管内组装两个内芯，成功地制造了一个带有分支的大型移植物，主动脉弓置换是在供体山羊中进行的，没有造成损害。总之,通过应用iBTA技术，我们让它成为可能，第一次,创建各种形状和设计的组织，使用现有的组织工程技术是困难的。更厚的iBTA诱导的组织表现出更高的断裂强度；然而，断裂应力与厚度成反比。这些发现拓宽了iBTA诱导的组织应用的范围。
