PDF(Pubmed)
Abstract：
Securing high-quality cell sources is important in regenerative medicine. In this study, we developed a device that can accumulate autologous stem cells in the body. When small wire-assembled molds were embedded in the dorsal subcutaneous pouches of beagles for several weeks, collagen-based tissues with minimal inflammation formed inside the molds. At 3 weeks of embedding, the outer areas of the tissues were composed of immature type III collagen with large amounts of cells expressing SSEA3 or SSEA4 markers, in addition to growth factors such as HGF or VEGF. When separated from the tissues by collagenase treatment, approximately four million cells with a proportion of 70% CD90-positive and 20% SSEA3- or SSEA4-positive cells were recovered from the single mold. The cells could differentiate into bone or cartilage cells. The obtained cell-containing tissues are expected to have potential as therapeutic materials or cell sources in regenerative medicine.
摘要：
确保高质量的细胞来源在再生医学中很重要。在这项研究中,我们开发了一种可以在体内积累自体干细胞的装置。当将小型金属丝组装的模具嵌入比格犬的背侧皮下袋中数周时，以胶原蛋白为基础的组织，在模具内部形成最小的炎症。在3周的嵌入，组织的外部区域由未成熟的III型胶原蛋白组成，大量细胞表达SSEA3或SSEA4标记，除了生长因子如HGF或VEGF。当通过胶原酶处理与组织分离时，从单个模具中回收约四百万个细胞,其比例为70%CD90阳性和20%SSEA3-或SSEA4-阳性细胞。细胞可以分化为骨骼或软骨细胞。预期所获得的含细胞组织在再生医学中具有作为治疗材料或细胞来源的潜力。
