PDF(Pubmed)
Abstract：
BACKGROUND: Currently, the Enterobacteriaceae species are responsible for a variety of serious infections and are already considered a global public health problem, especially in underdeveloped countries, where surveillance and monitoring programs are still scarce and limited. Analyses were performed on the complete genome of an extensively antibiotic-resistant strain of Enterobater hormaechei, which was isolated from a patient with non-Hodgkin\'s lymphoma, who had been admitted to a hospital in the city of Manaus, Brazil.
METHODS: Phenotypical identification and susceptibility tests were performed in automated equipment. Total DNA extraction was performed using the PureLink genomic DNA mini-Kit. The genomic DNA library was prepared with Illumina Microbial Amplicon Prep and sequenced in the MiSeq Illumina Platform. The assembly of the whole-genome and individual analyses of specific resistance genes extracted were carried out using online tools and the Geneious Prime software.
RESULTS: The analyses identified an extensively resistant ST90 clone of E. hormaechei carrying different genes, including blaCTX-M-15, blaGES-2, blaTEM-1A, blaACT-15, blaOXA-1 and blaNDM-1, [aac(3)-IIa, aac(6\')-Ian, ant(2″)-Ia], [aac(6\')-Ib-cr, (qnrB1)], dfrA25, sul1 and sul2, catB3, fosA, and qnrB, in addition to resistance to chlorhexidine, which is widely used in patient antisepsis.
CONCLUSIONS: These findings highlight the need for actions to control and monitor these pathogens in the hospital environment.
METHODS: Phenotypical identification and susceptibility tests were performed in automated equipment. Total DNA extraction was performed using the PureLink genomic DNA mini-Kit. The genomic DNA library was prepared with Illumina Microbial Amplicon Prep and sequenced in the MiSeq Illumina Platform. The assembly of the whole-genome and individual analyses of specific resistance genes extracted were carried out using online tools and the Geneious Prime software.
RESULTS: The analyses identified an extensively resistant ST90 clone of E. hormaechei carrying different genes, including blaCTX-M-15, blaGES-2, blaTEM-1A, blaACT-15, blaOXA-1 and blaNDM-1, [aac(3)-IIa, aac(6\')-Ian, ant(2″)-Ia], [aac(6\')-Ib-cr, (qnrB1)], dfrA25, sul1 and sul2, catB3, fosA, and qnrB, in addition to resistance to chlorhexidine, which is widely used in patient antisepsis.
CONCLUSIONS: These findings highlight the need for actions to control and monitor these pathogens in the hospital environment.
摘要：
背景：目前，肠杆菌科细菌是各种严重感染的原因，已经被认为是全球公共卫生问题，特别是在不发达国家，那里的监视和监控计划仍然很少和有限。对广泛耐药的肠杆菌hormaechei菌株的完整基因组进行了分析，从一名非霍奇金淋巴瘤患者中分离出来，他进了马瑙斯市的一家医院，巴西。
方法：在自动化设备中进行表型鉴定和药敏试验。使用PureLink基因组DNA迷你试剂盒进行总DNA提取。用Illumina微生物扩增子制备物制备基因组DNA文库,并在MiSeqIllumina平台中测序。使用在线工具和GeneiousPrime软件进行全基因组的组装和提取的特定抗性基因的个体分析。
结果：分析确定了携带不同基因的广泛抗性ST90E.hormaechei克隆，包括blaCTX-M-15,blaGES-2,blaTEM-1A,blaACT-15，blaOXA-1和blaNDM-1，[aac（3）-IIa，aac(6\')-Ian,蚂蚁(2″)-Ia]，[aac(6\')-Ib-cr，(qnrB1)],dfrA25,sul1和sul2,catB3,fosA,和qnrB，除了耐氯己定，广泛用于患者的防腐。
结论：这些发现强调了在医院环境中控制和监测这些病原体的需要。
方法：在自动化设备中进行表型鉴定和药敏试验。使用PureLink基因组DNA迷你试剂盒进行总DNA提取。用Illumina微生物扩增子制备物制备基因组DNA文库,并在MiSeqIllumina平台中测序。使用在线工具和GeneiousPrime软件进行全基因组的组装和提取的特定抗性基因的个体分析。
结果：分析确定了携带不同基因的广泛抗性ST90E.hormaechei克隆，包括blaCTX-M-15,blaGES-2,blaTEM-1A,blaACT-15，blaOXA-1和blaNDM-1，[aac（3）-IIa，aac(6\')-Ian,蚂蚁(2″)-Ia]，[aac(6\')-Ib-cr，(qnrB1)],dfrA25,sul1和sul2,catB3,fosA,和qnrB，除了耐氯己定，广泛用于患者的防腐。
结论：这些发现强调了在医院环境中控制和监测这些病原体的需要。
