PDF(Pubmed)
Abstract：
Heifer conception rate to the first service (HCR1) is defined as the number of heifers that become pregnant to the first breeding service compared to the heifers bred. This study aimed to identify loci associated and gene sets enriched for HCR1 for heifers that were bred by artificial insemination (AI, n = 2829) or were embryo transfer (ET, n = 2086) recipients, by completing a genome-wide association analysis and gene set enrichment analysis using SNP data (GSEA-SNP). Three unique loci, containing four positional candidate genes, were associated (p < 1 × 10-5) with HCR1 for ET recipients, while the GSEA-SNP identified four gene sets (NES ≥ 3) and sixty-two leading edge genes (LEGs) enriched for HCR1. While no loci were associated with HCR1 bred by AI, one gene set and twelve LEGs were enriched (NES ≥ 3) for HCR1 with the GSEA-SNP. This included one gene (PKD2) shared between HCR1 AI and ET services. Identifying loci associated or enriched for HCR1 provides an opportunity to use them as genomic selection tools to facilitate the selection of cattle with higher reproductive efficiency, and to better understand embryonic loss.
摘要：
首次服务的母牛受胎率（HCR1）定义为与繁殖的母牛相比，首次繁殖服务怀孕的母牛数量。这项研究旨在确定通过人工授精繁殖的小母牛的HCR1相关基因座和富集的基因集（AI，n=2829)或进行胚胎移植(ET，n=2086)收件人，通过使用SNP数据（GSEA-SNP）完成全基因组关联分析和基因集富集分析。三个独特的基因座，包含四个位置候选基因，与ET接受者的HCR1相关（p<1×10-5），而GSEA-SNP鉴定了四个基因集（NES≥3）和62个富集HCR1的前沿基因（LEGs）。虽然没有基因座与AI繁殖的HCR1相关，用GSEA-SNP富集HCR1的一个基因集和12个LEG(NES≥3)。这包括HCR1AI和ET服务之间共享的一个基因(PKD2)。确定与HCR1相关或富集的基因座提供了将它们用作基因组选择工具的机会，以促进选择具有更高繁殖效率的牛，更好地了解胚胎丢失。
