PDF(Pubmed)
Abstract：
Infections with gastrointestinal nematodes (GINs) reduce the economic efficiency of sheep operations and compromise animal welfare. Understanding the host\'s response to GIN infection can help producers identify animals that are naturally resistant to infection. The objective of this study was to characterize the hepatic transcriptome of sheep that had been naturally exposed to GIN parasites. The hepatic transcriptome was studied using RNA-Sequencing technology in animals characterized as high (n = 5) or medium (n = 6) based on their innate immune acute-phase (AP) response phenotype compared with uninfected controls (n = 4), and with biased antibody-mediated (AbMR, n = 5) or cell-mediated (CMR, n = 5) adaptive immune responsiveness compared to uninfected controls (n = 3). Following the assessment of sheep selected for innate responses, 0, 136, and 167 genes were differentially expressed (DE) between high- and medium-responding animals, high-responding and uninfected control animals, and medium-responding and uninfected control animals, respectively (false discovery rate (FDR) < 0.05, and fold change |FC| > 2). When adaptive immune responses were assessed, 0, 53, and 57 genes were DE between antibody- and cell-biased animals, antibody-biased and uninfected control animals, and cell-biased and uninfected control animals, respectively (FDR < 0.05, |FC| > 2). Functional analyses identified enriched gene ontology (GO) terms and metabolic pathways related to the innate immune response and energy metabolism. Six functional candidate genes were identified for further functional and validation studies to better understand the underlying biological mechanisms of host responses to GINs. These, in turn, can potentially help improve decision making and management practices to increase the overall host immune response to GIN infection.
摘要：
胃肠道线虫（GINs）感染降低了绵羊操作的经济效率，并损害了动物福利。了解宿主对GIN感染的反应可以帮助生产者识别自然抗感染的动物。这项研究的目的是表征天然暴露于GIN寄生虫的绵羊的肝脏转录组。使用RNA测序技术在动物中研究肝脏转录组，根据其先天免疫急性期（AP）反应表型，与未感染的对照（n=4）相比，高（n=5）或中等（n=6）。和偏倚抗体介导的(AbMR，n=5)或细胞介导的(CMR，n=5)与未感染对照相比的适应性免疫应答(n=3)。在对选择的绵羊进行先天反应评估后，0，136和167个基因在高和中等响应动物之间差异表达（DE），高反应和未感染的对照动物，和中等反应和未感染的对照动物，分别为(错误发现率(FDR)<0.05，倍数变化|FC|>2)。当评估适应性免疫反应时，0、53和57个基因在抗体和细胞偏向动物之间是DE，抗体偏倚和未感染的对照动物，和细胞偏向和未感染的对照动物，分别为(FDR<0.05，|FC|>2)。功能分析确定了与先天免疫反应和能量代谢相关的丰富的基因本体论（GO）术语和代谢途径。确定了六个功能候选基因用于进一步的功能和验证研究，以更好地了解宿主对GINs反应的潜在生物学机制。这些,反过来,可能有助于改善决策和管理实践，以增加宿主对GIN感染的整体免疫反应。
