PDF(Pubmed)
Abstract：
Orchids of the genus Paphiopedilum, also called slippers, are among the most valued representatives of the Orchidaceae family due to their aesthetic qualities. Due to overexploitation, deforestation, and illegal trade in these plants, especially in the vegetative phase, Paphiopedilum requires special protection. This genus is listed in Appendix I of the Convention on International Trade in Endangered Species of Wild Fauna and Flora. Their precise identification is of great importance for the preservation of genetic resources and biodiversity of the orchid family (Orchidaceae). Therefore, the main objective of the study was to investigate the usefulness of the DNA barcoding technique for the identification of endangered orchids of the genus Paphiopedilum and to determine the effectiveness of five loci: matK, rbcL, ITS2, atpF-atpH and trnH-psbA as potential molecular markers for species of this genus. Among single locus barcodes, matK was the most effective at identifying species (64%). Furthermore, matK, ITS2, matK + rbcL, and matK + trnH-psbA barcodes can be successfully used as a complementary tool to identify Paphiopedilum orchids while supporting morphological data provided by taxonomists.
摘要：
罂粟属的兰花，也叫拖鞋,由于其美学品质，它们是兰科最有价值的代表之一。由于过度开发,森林砍伐,这些植物的非法贸易，尤其是在植物生长阶段,蛇纹石需要特殊保护。该属列在《濒危野生动植物种国际贸易公约》附录I中。它们的精确鉴定对于保护兰花科（兰科）的遗传资源和生物多样性至关重要。因此,该研究的主要目的是研究DNA条形码技术对鉴定濒危兰花的有用性，并确定五个基因座的有效性：matK，rbcL,ITS2，atpF-atpH和trnH-psbA是该属物种的潜在分子标记。在单基因座条形码中，matK在识别物种方面最有效(64%)。此外，matK,ITS2,matK+rbcL,和matKtrnH-psbA条形码可以成功地用作补充工具来识别Paphiopedilum兰花，同时支持分类学家提供的形态数据。
