PDF(Pubmed)
Abstract：
Chondrocyte-based cell therapy has been used for more than 30 years and is still considered to be a promising method of cartilage repair despite some limitations. This review introduces the latest developments of four generations of autologous chondrocyte implantation and current autologous chondrocyte products. The regeneration of cartilage from adult chondrocytes is limited by culture-induced dedifferentiation and patient age. Cartibeads is an innovative three-step method to produce high-quality hyaline cartilage microtissues, and it is developed from adult dedifferentiated chondrocytes with a high number of cell passages. In addition, allogeneic chondrocyte therapies using the Quantum hollow-fiber bioreactor and several signaling pathways involved in chondrocyte-based cartilage repair are mentioned, such as WNT signaling, the BMP-2/WISP1 pathway, and the FGF19 pathway.
摘要：
基于软骨细胞的细胞疗法已经使用了30多年，尽管存在一些局限性，但仍被认为是一种有前途的软骨修复方法。本文综述了四代自体软骨细胞移植的最新进展以及目前自体软骨细胞产品的研究进展。从成年软骨细胞再生软骨受到培养诱导的去分化和患者年龄的限制。Cartibeads是一种创新的三步方法，可生产高质量的透明软骨微组织，它是从成体的去分化软骨细胞发展而来的，具有大量的细胞传代。此外,使用量子中空纤维生物反应器的同种异体软骨细胞治疗和参与软骨细胞修复的几个信号通路被提及，例如WNT信令，BMP-2/WISP1途径，和FGF19通路。
