PDF(Pubmed)
Abstract：
Over the last several years, there has been increased interest in cannabidiol (CBD) to treat various ailments such as pain, anxiety, insomnia, and inflammation. The potential for CBD as an anti-inflammatory therapy has come, in part, from its demonstrated ability to suppress neuroinflammation in autoimmune diseases, such as the mouse model of multiple sclerosis, experimental autoimmune encephalomyelitis (EAE). The increased use of CBD strongly suggests that more research is necessary to elucidate its safety and efficacy and determine the mechanisms by which it acts. Thus, we conducted two separate studies. In the first, RNA sequencing (RNA-Seq) analysis of brains of female mice undergoing EAE in the presence and absence of CBD was conducted to identify potential genes that mediated its neuroprotective effects when efficacious. In the second, we assessed some of the same genes in male and female mice treated with CBD in the absence of an immune stimulus. Together, these data showed that CBD modestly increased oxytocin (Oxt) and arginine vasopressin (vasopressin, Avp) gene expression in the brains of mice, regardless of whether there was active inflammation. Overall, these data suggest that Oxt and Avp might act as biomarkers for CBD exposure.
摘要：
在过去的几年里，人们对大麻二酚（CBD）治疗各种疾病如疼痛的兴趣越来越大，焦虑,失眠,和炎症。CBD作为抗炎疗法的潜力已经到来，在某种程度上,从其在自身免疫性疾病中抑制神经炎症的能力来看，比如多发性硬化症的小鼠模型，实验性自身免疫性脑脊髓炎（EAE）。CBD使用的增加强烈表明，有必要进行更多的研究来阐明其安全性和有效性，并确定其作用机制。因此，我们进行了两项独立的研究。在第一,进行在存在和不存在CBD的情况下经历EAE的雌性小鼠的脑的RNA测序(RNA-Seq)分析以鉴定当有效时介导其神经保护作用的潜在基因。在第二个，我们在没有免疫刺激的情况下，在接受CBD治疗的雄性和雌性小鼠中评估了一些相同的基因。一起,这些数据表明，CBD适度增加催产素（Oxt）和精氨酸加压素（加压素，Avp）小鼠大脑中的基因表达，不管是否有活动性炎症。总的来说,这些数据提示Oxt和Avp可能作为CBD暴露的生物标志物.
