PDF(Pubmed)
Abstract：
Hematoma clearance is critical for mitigating intracerebral hemorrhage (ICH)-induced brain injury. Multinucleated giant cells (MGCs), a type of phagocyte, and the complement system may play a pivotal role in hematoma resolution, but whether the complement system regulates MGC formation after ICH remains unclear. The current study investigated the following: (1) the characteristics of MGC formation after ICH, (2) whether it was impacted by complement C3 deficiency in mice and (3) whether it also influenced hematoma degradation (hemosiderin formation). Young and aged male mice, young female mice and C3-deficient and -sufficient mice received a 30 μL injection of autologous whole blood into the right basal ganglia. Brain histology and immunohistochemistry were used to examine MGC formation on days 3 and 7. Hemosiderin deposition was examined by autofluorescence on day 28. Following ICH, MGCs were predominantly located in the peri-hematoma region exhibiting multiple nuclei and containing red blood cells or their metabolites. Aging was associated with a decrease in MGC formation after ICH, while sex showed no discernible effect. C3 deficiency reduced MGC formation and reduced hemosiderin formation. Peri-hematomal MGCs may play an important role in hematoma resolution. Understanding how aging and complement C3 impact MGCs may provide important insights into how to regulate hematoma resolution.
摘要：
血肿清除对于减轻脑出血（ICH）引起的脑损伤至关重要。多核巨细胞（MGCs），一种吞噬细胞，补体系统可能在血肿消退中起关键作用，但补体系统是否调节ICH后的MGC形成尚不清楚。目前的研究调查了以下内容:(1)ICH后MGC的形成特征,（2）是否受到小鼠补体C3缺乏的影响；（3）是否也影响血肿降解（含铁血黄素形成）。年轻和年老的雄性老鼠，年轻的雌性小鼠和C3缺乏且足够的小鼠接受了30μL的自体全血注射到右基底神经节中。在第3天和第7天,使用脑组织学和免疫组织化学检查MGC形成。在第28天通过自发荧光检查铁血黄素沉积。在ICH之后，MGCs主要位于血肿周围区域，表现出多核并含有红细胞或其代谢物。老化与ICH后MGC形成的减少有关，而性别没有明显的影响。C3缺乏减少MGC形成和减少含铁血黄素形成。血肿周围MGCs可能在血肿消退中起重要作用。了解衰老和补体C3如何影响MGCs可能为如何调节血肿分辨率提供重要见解。
