PDF(Pubmed)
Abstract：
Fermented prebiotic and probiotic products with kefir are very important to slow down and prevent the growth of tumors and to treat cancer by stimulating the immune response against tumor cells. Cyclophosphamide (CPx) is widely preferred in cancer treatment but its effectiveness in high doses is restricted because of its side effects. The aim of this study was to investigate the protective effects of kefir against CPx-induced heart and liver toxicity. In an experiment, 42 Wistar albino rats were divided into six treatment groups: the control (Group 1), the group receiving 150 mg/kg CPx (Group 2), the groups receiving 5 and 10 mg/kg kefir (Groups 3 and 4) and the groups receiving 5 and 10 mg/kg kefir + CPx (Group 5 and 6). Fermented kefirs obtained on different days by traditional methods were mixed and given by gavage for 12 days, while a single dose of CPx was administered intraperitoneally (i.p.) on the 12th day of the experiment. It was observed that alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), creatinine kinase-MB (CK-MB), ischemia modified albumin (IMA) and Troponin I values, which indicate oxidative stress, increased in the CPx-administered group, and this level approached that of the control in the CPx + kefir groups. Likewise, as a result of the kefir, the rats\' CPx-induced histopathological symptoms were reduced, and their heart and liver tissue were significantly improved. In conclusion, it was observed that kefir had a cytoprotective effect against CPx-induced oxidative stress, hepatotoxicity and cardiotoxicity, bringing their biochemical parameters closer to those of the control by suppressing oxidative stress and reducing tissue damage.
摘要：
含有开菲尔的发酵益生元和益生菌产品对于减缓和预防肿瘤的生长以及通过刺激针对肿瘤细胞的免疫反应来治疗癌症非常重要。环磷酰胺（CPx）在癌症治疗中广泛优选，但由于其副作用，其在高剂量下的有效性受到限制。这项研究的目的是研究开菲尔对CPx引起的心脏和肝脏毒性的保护作用。在一个实验中,将42只Wistar白化病大鼠分为六个治疗组：对照组（第1组），接受150mg/kgCPx的组（第2组），接受5和10mg/kgkefir的组（第3和第4组）以及接受5和10mg/kgkefirCPx的组（第5和第6组）。将通过传统方法在不同日期获得的发酵克菲尔混合并通过管饲法给予12天，而在实验的第12天腹膜内（i.p.）施用单剂量的CPx。观察到丙氨酸转氨酶（ALT），天冬氨酸转氨酶（AST），碱性磷酸酶(ALP),乳酸脱氢酶（LDH），肌酐激酶-MB（CK-MB），缺血修饰白蛋白（IMA）和肌钙蛋白I值，这表明氧化应激，CPx给药组增加，在CPx开菲尔组中，这一水平接近对照组。同样，由于开菲尔，大鼠CPx诱导的组织病理学症状减轻，心脏和肝脏组织明显改善。总之,观察到开菲尔对CPx诱导的氧化应激具有细胞保护作用，肝毒性和心脏毒性，通过抑制氧化应激和减少组织损伤，使他们的生化参数更接近对照组。
