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Abstract：
Cotton is a globally significant economic crop. Brassinosteroids (BRs) are crucial to cotton development. This study systematically analyzed the BR synthase gene family in four cotton species and identified 60 BR genes: 20 in Gossypium hirsutum (GhBRs), 20 in G. barbadense (GbBRs), 10 in G. arboreum (GaBRs), and 10 in G. raimondii (GrBRs). The analysis was extended to chromosomal localization, evolutionary relationships, domain features, and cis-regulatory elements in the promoter regions of BR synthase genes. The results showed that the BR synthase genes were evenly distributed across different subgenomes and chromosomes. Bioinformatics analyses revealed high conservation of amino acid sequences, secondary structures, and conserved domains among the subfamily members, which is closely linked to their pivotal roles in the BR biosynthesis pathway. Cis-element distribution analysis of the BR synthase genes further underscored the complexity of BR gene expression regulation, which is influenced by multiple factors, including plant hormones, abiotic stress, and transcription factors. Expression profiling of GhBRs genes in various cotton tissues and developmental stages highlighted the key roles of GhROT3-1 and GhDET2-1 in fiber elongation and initiation, respectively. Protein-protein interactions and transcription factor analyses further elucidated the regulatory mechanisms of GhROT3-1 and GhDET2-1 in cotton growth and development. This study lays a theoretical foundation for understanding the role of the BR signaling pathway in cotton development, facilitating molecular breeding.
摘要：
棉花是全球重要的经济作物。油菜素类固醇（BRs）对棉花发育至关重要。本研究系统分析了4种棉种的BR合酶基因家族，并鉴定了60个BR基因：20个在陆地棉（GhBR）中，20在G.barbadense（GbBR）中，10在G.arboreum（GaBR）中，和10在G.raimondii（GrBRs）。分析扩展到染色体定位，进化关系，域特征,和BR合酶基因启动子区域的顺式调节元件。结果表明，BR合酶基因在不同的亚基因组和染色体上均匀分布。生物信息学分析揭示了氨基酸序列的高度保守性，二级结构,和亚家族成员之间的保守域，这与它们在BR生物合成途径中的关键作用密切相关。BR合酶基因的顺式元件分布分析进一步强调了BR基因表达调控的复杂性,这受到多种因素的影响，包括植物激素,非生物胁迫，和转录因子。GhBRs基因在不同棉花组织和发育阶段的表达谱强调了GhROT3-1和GhDET2-1在纤维伸长和起始中的关键作用，分别。蛋白质-蛋白质相互作用和转录因子分析进一步阐明了GhROT3-1和GhDET2-1在棉花生长发育中的调控机制。本研究为理解BR信号通路在棉花发育中的作用奠定了理论基础,促进分子育种。
