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Abstract：
Listeria monocytogenes, a foodborne pathogen causing listeriosis, poses substantial societal, economic, and public health challenges due to its resistance, persistence, and biofilm formation in the food industry. Exploring subinhibitory concentrations of compounds to target virulence inhibition and increase susceptibility to adverse conditions presents a promising strategy to mitigate its impact of L. monocytogenes and unveils new potential applications. Thus, this study aims to explore the effect of linalool on virulence factors of L. monocytogenes and potential use in the reduction in its tolerance to stressful conditions. This action was analysed considering the use of two sub-inhibitory concentrations of linalool, 0.312 and 0.625 mg/mL. We found that even with the lowest tested concentrations, a 65% inhibition of violacein production by Chromobacterium violaceum, 55% inhibition in biofilm formation by L. monocytogenes and 62% reduction on haemolysis caused by this bacterium were observed. In addition to its impact on virulence factors, linalool diminished the tolerance to osmotic stress (up to 4.3 log reduction after 24 h with 12% NaCl), as well as to high (up to 3.8 log reduction after 15 min at 55 °C) and low temperatures (up to 4.6 log reduction after 84 days with 12% NaCl at 4 °C). Thus, this study paves the way to further investigation into the potential utilization of linalool to mitigate the threat posed by L. monocytogenes in the field of food safety and public health.
摘要：
单核细胞增生李斯特菌,引起李斯特菌病的食源性病原体，实质性的社会姿态，经济,以及由于其抵制而带来的公共卫生挑战，持久性,以及食品工业中生物膜的形成。探索化合物的亚抑制浓度以靶向毒力抑制和增加对不利条件的敏感性为减轻其对单核细胞增生李斯特菌的影响提供了有希望的策略，并揭示了新的潜在应用。因此，本研究旨在探讨芳樟醇对单核细胞增生李斯特菌毒力因子的影响以及在降低其对应激条件的耐受性方面的潜在用途。考虑到使用两种亚抑制浓度的芳樟醇，对该作用进行了分析。0.312和0.625mg/mL。我们发现即使测试浓度最低,紫罗兰色杆菌对紫罗兰素生产的抑制作用为65%，观察到单核细胞增生李斯特菌对生物膜形成的抑制作用为55％，并且由该细菌引起的溶血减少了62％。除了对毒力因子的影响外，芳樟醇降低了对渗透胁迫的耐受性（在使用12％NaCl的24小时后降低了4.3log），以及高（在55°C下15分钟后降低3.8log）和低温（在4°C下用12％NaCl在84天后降低4.6log）。因此，这项研究为进一步研究芳樟醇的潜在利用，以减轻单核细胞增生李斯特菌在食品安全和公共卫生领域造成的威胁铺平了道路。
