PDF(Pubmed)
Abstract：
Silver has been shown to improve the antibiotic effects of other drugs against both Gram- positive and -negative bacteria. In this study, we investigated the antibiotic potential of cannabidiol (CBD), cannabichromene (CBC) and cannabigerol (CBG) and their acidic counterparts (CBDA, CBCA, CBGA) against Gram-positive bacteria and further explored the additive or synergistic effects of silver nitrate or silver nanoparticles using 96-well plate growth assays and viability (CFUs- colony-forming units). All six cannabinoids had strong antibiotic effects against MRSA with minimal inhibitory concentrations (MICs) of 2 mg/L for CBG, CBD and CBCA; 4 mg/L for CBGA; and 8 mg/L for CBC and CBDA. Using 96-well checkerboard assays, CBC, CBG and CBGA showed full or partial synergy with silver nitrate; CBC, CBDA and CBGA were fully synergistic with silver nanoparticles against MRSA. Using CFU assays, combinations of CBC, CBGA and CBG with either silver nitrate or silver nanoparticles, all at half or quarter MICs, demonstrated strong, time-dependent inhibition of bacterial growth (silver nitrate) and bactericidal effects (silver nanoparticles). These data will lead to further investigation into possible biomedical applications of specific cannabinoids in combination with silver salts or nanoparticles against drug-resistant Gram-positive bacteria.
摘要：
银已被证明可以改善其他药物对革兰氏阳性和阴性细菌的抗生素作用。在这项研究中,我们调查了大麻二酚（CBD）的抗生素潜力，大麻酚（CBC）和大麻酚（CBG）及其酸性对应物（CBDA，CBCA,CBGA）针对革兰氏阳性细菌，并使用96孔板生长测定和活力（CFU-菌落形成单位）进一步探索了硝酸银或银纳米颗粒的累加或协同作用。所有六种大麻素对MRSA具有很强的抗生素作用，对CBG的最低抑制浓度（MIC）为2mg/L，CBD和CBCA;CBGA为4mg/L;CBC和CBDA为8mg/L。使用96孔棋盘分析，CBC,CBG和CBGA与硝酸银表现出完全或部分协同作用；CBC，CBDA和CBGA与银纳米颗粒完全协同对抗MRSA。使用CFU测定，CBC的组合，CBGA和CBG与硝酸银或银纳米颗粒，全部为一半或四分之一的中等收入国家，表现出坚强，时间依赖性抑制细菌生长（硝酸银）和杀菌作用（银纳米颗粒）。这些数据将导致进一步研究特定大麻素与银盐或纳米颗粒组合对抗耐药革兰氏阳性细菌的可能的生物医学应用。
