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Abstract：
The production of nanomaterials through environmentally friendly methods is a top priority in the sustainable development of nanotechnology. This paper presents data on the synthesis of silver nanoparticles using an aqueous extract of Sphagnum fallax moss at room temperature. The morphology, stability, and size of the nanoparticles were analyzed using various techniques, including transmission electron microscopy, Doppler laser velocimetry, and UV-vis spectroscopy. In addition, Fourier transform infrared spectroscopy was used to analyze the presence of moss metabolites on the surface of nanomaterials. The effects of different concentrations of citrate-stabilized and moss extract-stabilized silver nanoparticles on cell viability, necrosis induction, and cell impedance were compared. The internalization of silver nanoparticles into both monolayers and three-dimensional cells spheroids was evaluated using dark-field microscopy and hyperspectral imaging. An eco-friendly method for the synthesis of silver nanoparticles at room temperature is proposed, which makes it possible to obtain spherical nanoparticles of 20-30 nm in size with high bioavailability and that have potential applications in various areas of human life.
摘要：
通过环境友好的方法生产纳米材料是纳米技术可持续发展的重中之重。本文介绍了在室温下使用泥炭藓的水提取物合成银纳米颗粒的数据。形态学,稳定性,使用各种技术分析纳米颗粒的大小，包括透射电子显微镜,多普勒激光测速,和紫外可见光谱。此外,采用傅里叶变换红外光谱分析纳米材料表面苔藓代谢物的存在。不同浓度的柠檬酸盐稳定和苔藓提取物稳定的银纳米颗粒对细胞活力的影响,坏死诱导，和细胞阻抗进行了比较。使用暗视野显微镜和高光谱成像评估了银纳米颗粒在单层和三维细胞球状体中的内化。提出了一种在室温下合成银纳米颗粒的环保方法，这使得可以获得具有高生物利用度的20-30nm大小的球形纳米颗粒，并且在人类生活的各个领域具有潜在的应用。
