Abstract：
OBJECTIVE: To investigate the relative expression level and clinical significance of LINC00475 in serum of patients with multiple myeloma (MM).
METHODS: The expression of LINC00475 in serum of 108 MM patients and five MM cell lines including RPMI 8226, NCI-H929, U266, OPM2 and CAG were detected by real-time fluorescence quantitative PCR. The diagnostic value of LINC00475 in MM was evaluated by receiver operating characteristic (ROC) curve analysis. The correlation of LINC00475 with patients\' characteristics was analyzed.
RESULTS: Compared with control groups, the expression of LINC00475 was up-regulated in serum of MM patients and MM cell lines (all P < 0.05). ROC curve analysis showed that the optimal cut-off value of LINC00475 was 262.4, the area under curve (AUC) was 0.924(95%CI : 0.884-0.964), and sensitivity and specificity was 83.3% and 91.7%, respectively, which indicated that LINC00475 had good evaluation value in MM patients. Compared with low-LINC00475 expression group, patients in high-LINC00475 expression group had higher levels of β2microglobulin (β2-MG) and Cystatin C (Cys-C) but lower albumin (ALB) (all P < 0.05). Compared with MM patients with International Staging System (ISS) stage I, the expression level of LINC00475 was significantly higher in patients with stage II and III (both P < 0.05).
CONCLUSIONS: LINC00475 is helpful to distinguish MM patients from healthy adults, which is correlated with the prognostic indicators such as β2-MG, ALB, and ISS stage.
UNASSIGNED: LINC00475在多发性骨髓瘤中的表达与临床意义.
UNASSIGNED: 探讨LINC00475在多发性骨髓瘤（MM）患者血清中的相对表达水平及临床意义。.
UNASSIGNED: 用实时荧光定量PCR技术检测108例MM患者血清及RPMI 8226、NCI-H929、U266、OPM2、CAG这5个MM细胞系中LINC00475的表达情况，受试者工作特征曲线评估其对MM的诊断价值，并结合患者的临床资料分析LINC00475与患者临床特征的关系。.
UNASSIGNED: 与健康对照组相比，MM患者血清及MM细胞系中LINC00475的表达明显上调（均P < 0.05）。受试者工作特征曲线分析显示，LINC00475的最佳截断值为262.4，曲线下面积为0.924(95%CI ：0.884-0.964)，灵敏度83.3%，特异度91.7%，表明LINC00475在MM患者中具有良好的评估价值。与LINC00475低表达组的MM患者相比，高表达组的患者具有较高水平的β2微球蛋白和胱抑素C（均P < 0.05），但白蛋白水平较低（P < 0.05）。与ISS分期Ⅰ期的MM患者相比，Ⅱ期和Ⅲ期患者LINC00475的表达水平均明显增高（均P < 0.05）。.
UNASSIGNED: 检测LINC00475的相对表达水平有助于区分MM患者和正常人群，其相对表达水平与MM患者的β2-微球蛋白、白蛋白、ISS分期等预后指标相关。.
METHODS: The expression of LINC00475 in serum of 108 MM patients and five MM cell lines including RPMI 8226, NCI-H929, U266, OPM2 and CAG were detected by real-time fluorescence quantitative PCR. The diagnostic value of LINC00475 in MM was evaluated by receiver operating characteristic (ROC) curve analysis. The correlation of LINC00475 with patients\' characteristics was analyzed.
RESULTS: Compared with control groups, the expression of LINC00475 was up-regulated in serum of MM patients and MM cell lines (all P < 0.05). ROC curve analysis showed that the optimal cut-off value of LINC00475 was 262.4, the area under curve (AUC) was 0.924(95%CI : 0.884-0.964), and sensitivity and specificity was 83.3% and 91.7%, respectively, which indicated that LINC00475 had good evaluation value in MM patients. Compared with low-LINC00475 expression group, patients in high-LINC00475 expression group had higher levels of β2microglobulin (β2-MG) and Cystatin C (Cys-C) but lower albumin (ALB) (all P < 0.05). Compared with MM patients with International Staging System (ISS) stage I, the expression level of LINC00475 was significantly higher in patients with stage II and III (both P < 0.05).
CONCLUSIONS: LINC00475 is helpful to distinguish MM patients from healthy adults, which is correlated with the prognostic indicators such as β2-MG, ALB, and ISS stage.
UNASSIGNED: LINC00475在多发性骨髓瘤中的表达与临床意义.
UNASSIGNED: 探讨LINC00475在多发性骨髓瘤（MM）患者血清中的相对表达水平及临床意义。.
UNASSIGNED: 用实时荧光定量PCR技术检测108例MM患者血清及RPMI 8226、NCI-H929、U266、OPM2、CAG这5个MM细胞系中LINC00475的表达情况，受试者工作特征曲线评估其对MM的诊断价值，并结合患者的临床资料分析LINC00475与患者临床特征的关系。.
UNASSIGNED: 与健康对照组相比，MM患者血清及MM细胞系中LINC00475的表达明显上调（均P < 0.05）。受试者工作特征曲线分析显示，LINC00475的最佳截断值为262.4，曲线下面积为0.924(95%CI ：0.884-0.964)，灵敏度83.3%，特异度91.7%，表明LINC00475在MM患者中具有良好的评估价值。与LINC00475低表达组的MM患者相比，高表达组的患者具有较高水平的β2微球蛋白和胱抑素C（均P < 0.05），但白蛋白水平较低（P < 0.05）。与ISS分期Ⅰ期的MM患者相比，Ⅱ期和Ⅲ期患者LINC00475的表达水平均明显增高（均P < 0.05）。.
UNASSIGNED: 检测LINC00475的相对表达水平有助于区分MM患者和正常人群，其相对表达水平与MM患者的β2-微球蛋白、白蛋白、ISS分期等预后指标相关。.
摘要：
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