PDF(Pubmed)
Abstract：
The development of nanomaterials has been speedily established in recent years, yet nanoparticles synthesized by traditional methods suffer unacceptable toxicity and the sustainability of the procedure for synthesizing such nanoparticles is inadequate. Consequently, green biosynthesis, which employs biopolymers, is gaining attraction as an environmentally sound alternative to less sustainable approaches. Chitosan-encapsulated nanoparticles exhibit exceptional antibacterial properties, offering a wide range of uses. Chitosan, obtained from shrimp shells, aided in the environmentally friendly synthesis of high-purity zinc oxide nanoparticles (ZnO NPs) with desirable features such as the extraction yield (41%), the deacetylation (88%), and the crystallinity index (74.54%). The particle size of ZnO NPs was 12 nm, while that of chitosan-ZnO NPs was 21 nm, and the bandgap energies of these nanomaterials were 3.98 and 3.48, respectively. The strong antibacterial action was demonstrated by ZnO NPs, chitosan-ZnO NPs, and chitosan-ZnO/PVP, particularly against Gram-positive bacteria, making them appropriate for therapeutic use. The photocatalytic degradation abilities were also assessed for all nanoparticles. At a concentration of 6 × 10-5 M, chitosan removed 90.5% of the methylene blue (MB) dye, ZnO NPs removed 97.4%, chitosan-coated ZnO NPs removed 99.6%, while chitosan-ZnO/PVP removed 100%. In the case of toluidine blue (TB), at a concentration of 4 × 10-3 M, the respective efficiencies were 96.8%, 96.8%, 99.5%, and 100%, respectively. Evaluation of radical scavenger activity revealed increased scavenging of ABTS and DPPH radicals by chitosan-ZnO/PVP compared to individual zinc oxide or chitosan-ZnO, where the IC50 results were 0.059, 0.092, 0.079 mg/mL, respectively, in the ABTS test, and 0.095, 0.083, 0.061, and 0.064 mg/mL in the DPPH test, respectively. Moreover, in silico toxicity studies were conducted to predict the organ-specific toxicity through ProTox II software. The obtained results suggest the probable safety and the absence of organ-specific toxicity with all the tested samples.
摘要：
近年来纳米材料的发展迅速,然而,通过传统方法合成的纳米颗粒遭受不可接受的毒性,并且用于合成这种纳米颗粒的程序的可持续性不足。因此,绿色生物合成，使用生物聚合物，作为一种不那么可持续的方法的无害环境替代方案，正在获得吸引力。壳聚糖包裹的纳米颗粒表现出优异的抗菌性能，提供了广泛的用途。壳聚糖,从虾壳中获得，有助于环境友好的高纯度氧化锌纳米颗粒（ZnONPs）的合成，具有理想的特征，如提取率（41％），脱乙酰（88%），和结晶度指数(74.54%)。ZnONPs的粒径为12nm，而壳聚糖-ZnONPs的含量为21nm，这些纳米材料的带隙能量分别为3.98和3.48。ZnONPs具有很强的抗菌作用，壳聚糖-ZnO纳米粒子，和壳聚糖-ZnO/PVP，特别是针对革兰氏阳性细菌，使它们适合治疗用途。还评估了所有纳米颗粒的光催化降解能力。浓度为6×10-5M时，壳聚糖去除90.5%的亚甲基蓝(MB)染料，ZnONPs去除97.4%，壳聚糖涂层ZnONPs去除99.6%，而壳聚糖-ZnO/PVP去除100%。在甲苯胺蓝（TB）的情况下，浓度为4×10-3M，各自的效率为96.8%，96.8%,99.5%,100%,分别。自由基清除剂活性的评估表明，与单个氧化锌或壳聚糖-ZnO相比，壳聚糖-ZnO/PVP对ABTS和DPPH自由基的清除增加，其中IC50结果为0.059、0.092、0.079mg/mL，分别,在ABTS测试中，DPPH试验中的0.095、0.083、0.061和0.064mg/mL，分别。此外，通过ProToxII软件进行计算机毒性研究以预测器官特异性毒性。获得的结果表明，所有测试样品都可能具有安全性，并且没有器官特异性毒性。
