PDF(Pubmed)
Abstract：
In electroretinographic (ERG) recordings of zebrafish, the light stimulus is usually delivered by a fiber optic cable. The purpose of this study was to determine whether the angle of incidence of the stimulus light from the fiber optic cable will affect the amplitudes and implicit times of the ERGs of zebrafish larvae. The larvae were positioned on their side with the right eye pointed upward. The light stimuli were delivered by a fiber optic cable from three directions of the larvae: frontal 0° (F0°), dorsal 30°(D30°), and ventral 30°(V30°). Photopic ERGs were recorded from 16 larvae at age 5-6 days post-fertilization. Our results showed that the mean amplitude of the b-wave elicited at D30° and V30° stimulation was significantly smaller than that elicited at F0° stimulation (P = 0.014 and P = 0.019, respectively). In addition, the mean amplitude of the d-wave elicited at D30° and V30° stimulation was significantly smaller than that elicited at F0° stimulation (P < 0.0001 and P = 0.015, respectively). However, the difference between the b-wave amplitudes elicited at D30° and V30° stimuli were not significant (P = 0.98), and the d-wave amplitudes were also not significantly different (P = 0.20). The average b-wave amplitudes elicited at D30° stimulation was 84.6 ± 15.7% and V30° stimulation was 84.8 ± 17.4% relative to that of F0° stimulation. The average d-wave amplitudes elicited by D30° stimulation was 85.5 ± 15.2% and by V30° stimulation was 79.0 ± 11.0% relative to that of F0° stimulation. The differences in the implicit times of the b- and d-wave elicited by the different directions of stimulation were not significant (P = 0.52 and P = 0.14, respectively). We conclude that the amplitude of the photopic ERGs is affected by the angle of the incident light. Thus, it would be better to use ganzfeld stimuli to elicit maximum b- and d-wave amplitudes of the photopic ERGs of zebrafish larvae.
摘要：
在斑马鱼的视网膜电图（ERG）记录中，光刺激通常由光纤电缆传递。这项研究的目的是确定来自光纤电缆的刺激光的入射角是否会影响斑马鱼幼虫ERG的振幅和隐含时间。幼虫被定位在它们的侧面,右眼向上指向。光刺激通过光纤电缆从幼虫的三个方向传递：正面0°（F0°），背侧30°(D30°),腹侧30°(V30°)。在受精后5-6天的年龄从16个幼虫中记录了照相ERG。我们的结果表明，在D30°和V30°刺激下引起的b波的平均振幅明显小于在F0°刺激下引起的b波的平均振幅（分别为P=0.014和P=0.019）。此外,在D30°和V30°刺激下引起的d波的平均振幅显着小于在F0°刺激下引起的平均振幅（分别为P<0.0001和P=0.015）。然而,在D30°和V30°刺激引起的b波振幅之间的差异不显著(P=0.98)。d波振幅也没有显着差异（P=0.20）。相对于F0°刺激，D30°刺激引起的平均b波振幅为84.6±15.7％，V30°刺激为84.8±17.4％。相对于F0°刺激，D30°刺激引起的平均d波振幅为85.5±15.2％，V30°刺激引起的平均d波振幅为79.0±11.0％。不同方向刺激引起的b波和d波隐含时间差异不显著(分别为P=0.52和P=0.14)。我们得出的结论是，适光ERG的幅度受入射光角度的影响。因此，最好使用ganzfeld刺激引起斑马鱼幼虫明视ERG的最大b波和d波振幅。
