Abstract：
The effects of adding vinasse (VIN) as a co-substrate on the stability and production of volatile fatty acids (VFA) and methane (CH4) during the anaerobic digestion (AD) of microalgal biomass (MB) were evaluated. The AD system consisted of an acidogenic reactor (AR) followed by a methanogenic reactor (MR). The experiment was divided into phase I-start-up and AD of VIN; phase II-MB+VIN co-digestion (50:50 based on chemical oxygen demand (COD)); and phase III-co-digestion of pretreated MB and VIN (PTMB+VIN, 50:50). In phase I, the total amount of VFA in the AR increased from 240 to 2126 mg/L. In the MR, the conversion of VFA into CH4 yielded an average of 71 ± 37 NmL CH4/g CODin. In phase II, the initial CH4 production was 246 ± 31 mL CH4/g CODin but it decreased to 63 mL CH4/g CODin due to the accumulation of longer chain acids. More stable conditions were achieved after two hydraulic retention cycles and the average CH4 yield in this phase was 183 mL CH4/g CODin. In phase III, when using PTMB, 197 ± 72 NmL CH4/g CODin were obtained, i.e., a 2.7- and 1.1-fold increases compared to phases I and II, respectively. The predominance of acetate producers and syntrophic organisms suggests acetoclastic methanogenesis, confirmed by the occurrence of Methanosaeta (10.5%).
摘要：
评估了在微藻生物质（MB）的厌氧消化（AD）过程中添加酒糟（VIN）作为共底物对挥发性脂肪酸（VFA）和甲烷（CH4）的稳定性和产生的影响。AD系统由产酸反应器(AR)和随后的产甲烷反应器(MR)组成。实验分为VIN的I期启动和AD；II期MBVIN共消化（基于化学需氧量（COD）为50：50）；以及预处理的MB和VIN（PTMBVIN，50:50)。在第一阶段,AR中VFA的总量从240增加到2126mg/L。在MR中，VFA转化为CH4的平均值为71±37NmLCH4/gCOD。在第二阶段,最初的CH4产量为246±31mLCH4/gCODin，但由于长链酸的积累，其降至63mLCH4/gCODin。在两个水力保留循环之后获得更稳定的条件,并且该阶段中的平均CH4产率为183mLCH4/gCOD。在第三阶段，使用PTMB时，获得197±72NmLCH4/gCODin，即，与第一阶段和第二阶段相比，增加了2.7倍和1.1倍，分别。乙酸产生者和互养生物的优势表明，乙酸分解甲烷的生成，确认为甲烷的发生（10.5％）。
