PDF(Pubmed)
Abstract：
Phytochemicals isolated from essential oils are effective alternatives for inhibiting microbial pathogens. Bovine protothecal mastitis is the cause of a reduction in milk production and the secretion of thin, watery milk with white flakes. In the present study, we performed in vitro susceptibility testing of the phytochemicals carvacrol, citral, and thymol in Prototheca strains isolated from cases of protothecosis in small animals and cow feces. The susceptibility of the algae to carvacrol, citral, and thymol was assessed using the modified CLSI M27-A3 broth microdilution method. The ranges of the minimum inhibitory concentrations (MIC%) of the phytochemicals in all isolates were 0.03% to 0.125% for carvacrol, 0.03% to 0.25% for citral, and 0.06% to 0.25% for thymol. Based on these results, carvacrol, citral, and thymol appear effective against Prototheca species at the tested concentrations, and may thus be useful for environmental disinfection in barns.
摘要：
从精油中分离的植物化学物质是抑制微生物病原体的有效替代品。牛原鞘性乳腺炎是导致产奶量减少和分泌稀薄的原因，白色薄片的含水牛奶。在本研究中,我们进行了植物化学物质香芹酚的体外药敏试验，柠檬醛,从小动物和牛粪便中的原虫病病例中分离出的原虫菌株和百里酚。藻类对香芹酚的敏感性,柠檬醛,和百里酚使用改良的CLSIM27-A3肉汤微量稀释法进行评估。所有分离物中植物化学物质的最小抑制浓度（MIC％）范围为香芹酚的0.03％至0.125％，柠檬醛为0.03%至0.25%，百里酚为0.06％至0.25％。基于这些结果,香芹酚,柠檬醛,百里酚在测试浓度下对原藻物种有效，因此可用于谷仓的环境消毒。
