Abstract：
UNASSIGNED: Dentin hypersensitivity (DH) is a prevalent condition, but long-term effective treatments are scarce. Differentiation of odontoblast-like cells is promising for inducing tertiary dentinogenesis and ensuring sustained therapeutic efficacy against DH. This study examined the effects and mechanism of action of mild heat stress (MHS) on the differentiation of odontoblast-like MDPC-23 cells.
UNASSIGNED: We used a heating device to accurately control the temperature and duration, mimicking the thermal microenvironment of odontoblast-like cells. Using this device, the effects of MHS on cell viability and differentiation were examined. Cell viability was assessed using the MTT assay. The expression and nucleoplasmic ratio of the yes-associated protein (YAP) were examined by western blotting and immunofluorescence. The gene expression levels of heat shock proteins (HSPs) and dentin matrix protein-1 (DMP1) were measured using qPCR. Dentin sialophosphoprotein (DSPP) expression was evaluated using immunofluorescence and immunoblotting. Verteporfin was used to inhibit YAP activity.
UNASSIGNED: Mild heat stress (MHS) enhanced the odontoblast differentiation of MDPC-23 cells while maintaining cell viability. MHS also increased YAP activity, as well as the levels of HSP25 mRNA, HSP70 mRNA, HSP90α mRNA, DMP1 mRNA, and DSPP protein. However, after YAP inhibition, both cell viability and the levels of HSP90α mRNA, DMP1 mRNA, and DSPP protein were reduced.
UNASSIGNED: YAP plays a crucial role in maintaining cell viability and promoting odontoblast differentiation of MDPC-23 cells under MHS. Consequently, MHS is a potential therapeutic strategy for DH, and boosting YAP activity could be beneficial for maintaining cell viability and promoting odontoblast differentiation.
UNASSIGNED: We used a heating device to accurately control the temperature and duration, mimicking the thermal microenvironment of odontoblast-like cells. Using this device, the effects of MHS on cell viability and differentiation were examined. Cell viability was assessed using the MTT assay. The expression and nucleoplasmic ratio of the yes-associated protein (YAP) were examined by western blotting and immunofluorescence. The gene expression levels of heat shock proteins (HSPs) and dentin matrix protein-1 (DMP1) were measured using qPCR. Dentin sialophosphoprotein (DSPP) expression was evaluated using immunofluorescence and immunoblotting. Verteporfin was used to inhibit YAP activity.
UNASSIGNED: Mild heat stress (MHS) enhanced the odontoblast differentiation of MDPC-23 cells while maintaining cell viability. MHS also increased YAP activity, as well as the levels of HSP25 mRNA, HSP70 mRNA, HSP90α mRNA, DMP1 mRNA, and DSPP protein. However, after YAP inhibition, both cell viability and the levels of HSP90α mRNA, DMP1 mRNA, and DSPP protein were reduced.
UNASSIGNED: YAP plays a crucial role in maintaining cell viability and promoting odontoblast differentiation of MDPC-23 cells under MHS. Consequently, MHS is a potential therapeutic strategy for DH, and boosting YAP activity could be beneficial for maintaining cell viability and promoting odontoblast differentiation.
摘要：
■牙本质超敏反应（DH）是一种普遍的疾病，但是长期有效的治疗方法很少。成牙本质细胞样细胞的分化有望诱导三级牙本质生成并确保针对DH的持续治疗功效。本研究研究了轻度热应激（MHS）对成牙本质细胞样MDPC-23细胞分化的影响和作用机制。
■我们使用了加热装置来精确控制温度和持续时间，模拟成牙本质细胞样细胞的热微环境。使用这个设备，研究了MHS对细胞活力和分化的影响。使用MTT测定评估细胞活力。通过蛋白质印迹和免疫荧光检查Yes相关蛋白（YAP）的表达和核质比。使用qPCR测量热休克蛋白（HSP）和牙本质基质蛋白1（DMP1）的基因表达水平。使用免疫荧光和免疫印迹评估牙本质唾液酸糖蛋白（DSPP）的表达。Verteporfin用于抑制YAP活性。
■轻度热应激（MHS）增强了MDPC-23细胞的成牙本质细胞分化，同时保持了细胞活力。MHS还增加了YAP活动，以及HSP25mRNA的水平，HSP70mRNA,HSP90αmRNA,DMP1mRNA,和DSPP蛋白。然而,YAP抑制后，细胞活力和HSP90αmRNA水平，DMP1mRNA,和DSPP蛋白减少。
■YAP在MHS下维持MDPC-23细胞的活力和促进成牙本质细胞分化中起着至关重要的作用。因此,MHS是DH的潜在治疗策略，和提高YAP活性可能有利于维持细胞活力和促进成牙本质细胞分化。
■我们使用了加热装置来精确控制温度和持续时间，模拟成牙本质细胞样细胞的热微环境。使用这个设备，研究了MHS对细胞活力和分化的影响。使用MTT测定评估细胞活力。通过蛋白质印迹和免疫荧光检查Yes相关蛋白（YAP）的表达和核质比。使用qPCR测量热休克蛋白（HSP）和牙本质基质蛋白1（DMP1）的基因表达水平。使用免疫荧光和免疫印迹评估牙本质唾液酸糖蛋白（DSPP）的表达。Verteporfin用于抑制YAP活性。
■轻度热应激（MHS）增强了MDPC-23细胞的成牙本质细胞分化，同时保持了细胞活力。MHS还增加了YAP活动，以及HSP25mRNA的水平，HSP70mRNA,HSP90αmRNA,DMP1mRNA,和DSPP蛋白。然而,YAP抑制后，细胞活力和HSP90αmRNA水平，DMP1mRNA,和DSPP蛋白减少。
■YAP在MHS下维持MDPC-23细胞的活力和促进成牙本质细胞分化中起着至关重要的作用。因此,MHS是DH的潜在治疗策略，和提高YAP活性可能有利于维持细胞活力和促进成牙本质细胞分化。
