Abstract：
Lead (Pb(II)) is a pervasive heavy metal toxin with many well-established negative effects on human health. Lead toxicity arises from cumulative, repeated environmental exposures. Thus, prophylactic strategies to protect against the bioaccumulation of lead could reduce lead-associated human pathologies. Here we show that DNA and RNA aptamers protect C. elegans from toxic phenotypes caused by lead. Reproductive toxicity, as measured by brood size assays, is prevented by co-feeding of animals with DNA or RNA aptamers. Similarly, lead-induced neurotoxicity, measured by behavioral assays, are also normalized by aptamer feeding. Further, cultured human HEK293 and primary murine osteoblasts are protected from lead toxicity by transfection with DNA aptamers. The osteogenic development, which is decreased by lead exposure, is maintained by prior transfection of lead-binding DNA aptamers. Aptamers may be an effective strategy for the protection of human health in the face of increasing environmental toxicants.
摘要：
铅（Pb（II））是一种普遍存在的重金属毒素，对人体健康具有许多公认的负面影响。铅毒性来自累积，重复的环境暴露。因此，预防铅生物蓄积的预防策略可以减少与铅相关的人类病理。在这里，我们表明DNA和RNA适体保护秀丽隐杆线虫免受铅引起的毒性表型。生殖毒性,通过育卵大小测定法测量，通过用DNA或RNA适体共同喂养动物来预防。同样，铅引起的神经毒性，通过行为测定法测量，也通过适体喂养进行归一化。Further,通过用DNA适体转染保护培养的人HEK293和原代鼠成骨细胞免受铅毒性。成骨发育，铅暴露减少了，通过先前转染结合铅的DNA适体来维持。面对日益增加的环境毒物，适体可能是保护人类健康的有效策略。
