Abstract：
Increasing incidences of fungal infections and prevailing antifungal resistance in healthcare settings has given rise to an antifungal crisis on a global scale. The members of the genus Candida, owing to their ability to acquire sessile growth, are primarily associated with superficial to invasive fungal infections, including the implant-associated infections. The present study introduces a novel approach to combat the sessile/biofilm growth of Candida by fabricating nanofibers using a nanoencapsulation approach. This technique involves the synthesis of tyrosol (TYS) functionalized chitosan gold nanocomposite, which is then encapsulated into PVA/AG polymeric matrix using electrospinning. The FESEM, FTIR analysis of prepared TYS-AuNP@PVA/AG NF suggested the successful encapsulation of TYS into the nanofibers. Further, the sustained and long-term stability of TYS in the medium was confirmed by drug release and storage stability studies. The prepared nanomats can absorb the fluid, as evidenced by the swelling index of the nanofibers. The growth and biofilm inhibition, as well as the disintegration studies against Candida, showed 60-70 % biofilm disintegration when 10 mg of TYS-AuNP@PVA/AG NF was used, hence confirming its biological effectiveness. Subsequently, the nanofibers considerably reduced the hydrophobicity index and ergosterol content of the treated cells. Considering the challenges associated with the inhibition/disruption of fungal biofilm, the fabricated nanofibers prove their effectiveness against Candida biofilm. Therefore, nanocomposite-loaded nanofibers have emerged as potential materials that can control fungal colonization and could also promote healing.
摘要：
在医疗机构中，真菌感染的发病率和普遍的抗真菌耐药性的增加导致了全球范围内的抗真菌危机。念珠菌属的成员，由于它们获得固着生长的能力，主要与浅层到侵入性真菌感染有关，包括植入物相关的感染。本研究介绍了一种通过使用纳米封装方法制造纳米纤维来对抗念珠菌的固着/生物膜生长的新方法。该技术涉及合成酪醇（TYS）功能化的壳聚糖金纳米复合材料，然后使用静电纺丝将其包封到PVA/AG聚合物基质中。FESEM，制备的TYS-AuNP@PVA/AGNF的FTIR分析表明TYS成功包封到纳米纤维中。Further,药物释放和储存稳定性研究证实了TYS在培养基中的持续和长期稳定性。制备的纳米粒子可以吸收流体，纳米纤维的溶胀指数证明了这一点。生长和生物膜抑制，以及针对念珠菌的崩解研究，当使用10mg的TYS-AuNP@PVA/AGNF时，显示60-70％的生物膜崩解，从而证实了它的生物有效性。随后，纳米纤维大大降低了处理细胞的疏水性指数和麦角甾醇含量。考虑到与真菌生物膜的抑制/破坏相关的挑战，制造的纳米纤维证明了它们对念珠菌生物膜的有效性。因此,纳米复合材料负载的纳米纤维已成为潜在的材料，可以控制真菌定植，也可以促进愈合。
