Abstract：
OBJECTIVE: To study the differences in immune cell profiles in uterine fibroids (Fibs) and matched myometrium (Myo).
METHODS: Observational study.
METHODS: Laboratory study.
METHODS: The study included tissue that was collected from 10 pairs of Fib and matched Myo from women, not on hormonal medications, undergoing hysterectomy and myomectomy.
METHODS: None.
METHODS: Differences in immune cell and cytokine composition between Fib and matched Myo.
RESULTS: The mass cytometry analysis indicated that Fibs had a significantly higher number of natural killer (NK) cells, total macrophages, M2 macrophages, and conventional dendritic cells when compared with matched Myo from the same patient. In contrast, Fibs had significantly fewer CD3 and CD4 T cells when compared with Myo. The mass cytometry analysis results did not show any significant difference in the number of resting mast cells. Immunoflurorescent and immunohistochemical imaging confirmed the cytometry by time of flight results, showing a significantly higher number of NK cells, tryptase-positive mast cells indicative of mast cell activation, total macrophages, and M2 cells in Fibs and a significantly lower number of CD3 and CD4 T cells. The cytokine assay revealed significantly increased levels of human interferon α2, interleukin (IL)-1α, and platelet-derived growth factor AA and significantly lower levels of macrophage colony-stimulating factor and IL-1 receptor antagonist in Fib.
CONCLUSIONS: Our results show significant differences in immune cell populations and cytokine levels between Fib and Myo. These differences could account for the increased inflammation in fib and a potential mechanism by which these tumors evade the immune system. These findings provide a foundation for further studies exploring the role of immune cells in Fib development.
METHODS: Observational study.
METHODS: Laboratory study.
METHODS: The study included tissue that was collected from 10 pairs of Fib and matched Myo from women, not on hormonal medications, undergoing hysterectomy and myomectomy.
METHODS: None.
METHODS: Differences in immune cell and cytokine composition between Fib and matched Myo.
RESULTS: The mass cytometry analysis indicated that Fibs had a significantly higher number of natural killer (NK) cells, total macrophages, M2 macrophages, and conventional dendritic cells when compared with matched Myo from the same patient. In contrast, Fibs had significantly fewer CD3 and CD4 T cells when compared with Myo. The mass cytometry analysis results did not show any significant difference in the number of resting mast cells. Immunoflurorescent and immunohistochemical imaging confirmed the cytometry by time of flight results, showing a significantly higher number of NK cells, tryptase-positive mast cells indicative of mast cell activation, total macrophages, and M2 cells in Fibs and a significantly lower number of CD3 and CD4 T cells. The cytokine assay revealed significantly increased levels of human interferon α2, interleukin (IL)-1α, and platelet-derived growth factor AA and significantly lower levels of macrophage colony-stimulating factor and IL-1 receptor antagonist in Fib.
CONCLUSIONS: Our results show significant differences in immune cell populations and cytokine levels between Fib and Myo. These differences could account for the increased inflammation in fib and a potential mechanism by which these tumors evade the immune system. These findings provide a foundation for further studies exploring the role of immune cells in Fib development.
摘要：
目的：研究子宫肌瘤（Fib）和匹配的子宫肌层（Myo）中免疫细胞谱的差异。
方法：观察性研究设置：实验室研究患者：该研究包括从女性的十对肌瘤和匹配的子宫肌层收集的组织，不是荷尔蒙药物，行子宫切除术和子宫肌瘤切除术。
方法：无主要结果指标：肌瘤和匹配的子宫肌层之间免疫细胞和细胞因子组成的差异总巨噬细胞，M2巨噬细胞，与来自同一患者的匹配子宫肌层相比，以及常规树突状细胞。相比之下,与子宫肌层相比，肌瘤的CD3和CD4T细胞明显减少。质量细胞计数分析未显示静息肥大细胞数量的任何显着差异。IFC和IHC成像证实了CytoF结果，显示显著较高(p<0.05)数量的NK，类胰蛋白酶阳性的肥大细胞表明肥大细胞活化，总巨噬细胞，和M2细胞中的肌瘤和显著较低(p<0.05)数目的CD3和CD4T细胞。细胞因子测定显示IFNA2，IL-1α的水平显着增加（p<0.05），和PDGF-AA，并显着降低Fib中M-CSF和IL-1RA的水平。
结论：我们的结果显示Fib和Myo之间的免疫细胞群体和细胞因子水平存在显著差异。巨噬细胞总数显著增加，M2巨噬细胞，NK细胞,和树突状细胞以及Fib中CD3和CD4T细胞的显着减少。IHC证实静息肥大细胞总数无差异，但是Fib中的类胰蛋白酶阳性肥大细胞显着增加。Fibs也表达显著较高水平的IFNA2、IL-1α、和PDGF-AA，与匹配的子宫肌层相比，IL-1RA和M-CSF的水平显着降低。这些发现为进一步研究探索免疫细胞在Fib发育中的作用奠定了基础。
方法：观察性研究设置：实验室研究患者：该研究包括从女性的十对肌瘤和匹配的子宫肌层收集的组织，不是荷尔蒙药物，行子宫切除术和子宫肌瘤切除术。
方法：无主要结果指标：肌瘤和匹配的子宫肌层之间免疫细胞和细胞因子组成的差异总巨噬细胞，M2巨噬细胞，与来自同一患者的匹配子宫肌层相比，以及常规树突状细胞。相比之下,与子宫肌层相比，肌瘤的CD3和CD4T细胞明显减少。质量细胞计数分析未显示静息肥大细胞数量的任何显着差异。IFC和IHC成像证实了CytoF结果，显示显著较高(p<0.05)数量的NK，类胰蛋白酶阳性的肥大细胞表明肥大细胞活化，总巨噬细胞，和M2细胞中的肌瘤和显著较低(p<0.05)数目的CD3和CD4T细胞。细胞因子测定显示IFNA2，IL-1α的水平显着增加（p<0.05），和PDGF-AA，并显着降低Fib中M-CSF和IL-1RA的水平。
结论：我们的结果显示Fib和Myo之间的免疫细胞群体和细胞因子水平存在显著差异。巨噬细胞总数显著增加，M2巨噬细胞，NK细胞,和树突状细胞以及Fib中CD3和CD4T细胞的显着减少。IHC证实静息肥大细胞总数无差异，但是Fib中的类胰蛋白酶阳性肥大细胞显着增加。Fibs也表达显著较高水平的IFNA2、IL-1α、和PDGF-AA，与匹配的子宫肌层相比，IL-1RA和M-CSF的水平显着降低。这些发现为进一步研究探索免疫细胞在Fib发育中的作用奠定了基础。
