Abstract：
The treatment of diabetic wounds possessed significant challenges in clinical practice, which was accompanied with continuous infection, inflammation, and limited angiogenesis. Current wound dressings used for diabetic wound healing struggle to address these issues simultaneously. Therefore, Ga3+ was added to the chitosan/silk solution to confer potent antibacterial properties. Subsequently, umbilical cord mesenchymal stem cell exosomes (UCSC-Exo) were integrated into the gallium/chitosan/silk solution to enhance its angiogenesis-inducing activity. The mixture was lyophilized to prepare gallium/chitosan/silk/exosome sponge scaffolds (Ga/CSSF-Exo sponge scaffolds). The experiments of In vitro and in vivo demonstrated that Ga/CSSF-Exo sponge scaffolds exhibited sustained release of Ga3+ and bioactive exosomes, which effectively exerted continuous antibacterial effects and promoted angiogenesis. In diabetic rat wound models, Ga/CSSF-Exo sponge scaffolds facilitated angiogenesis, suppressed bacterial growth and inflammation, as well as promoted collagen deposition and re-epithelialization of wounds. Collectively, our findings suggested that Ga/CSSF-Exo held excellent potential for diabetic wound healing.
摘要：
糖尿病创面的治疗在临床实践中具有重大挑战,伴随着持续的感染，炎症,和有限的血管生成。用于糖尿病伤口愈合的当前伤口敷料努力同时解决这些问题。因此,将Ga3+添加到壳聚糖/丝溶液中以赋予有效的抗菌性能。随后，将脐带间充质干细胞外泌体（UCSC-Exo）整合到镓/壳聚糖/丝溶液中以增强其血管生成诱导活性。将混合物冻干以制备镓/壳聚糖/丝/外泌体海绵支架(Ga/CSSF-Exo海绵支架)。体外和体内实验表明，Ga/CSSF-Exo海绵支架表现出持续释放的Ga3+和生物活性外泌体，有效发挥持续抗菌作用，促进血管生成。在糖尿病大鼠伤口模型中，Ga/CSSF-Exo海绵支架促进血管生成，抑制细菌生长和炎症，以及促进胶原沉积和伤口的再上皮化。总的来说，我们的研究结果表明,Ga/CSSF-Exo对糖尿病伤口愈合具有极好的潜力.
