Abstract：
Microbial colonization and development of infections in wounds is a sign of chronicity. The prevailing approach to manage and treat these wounds involves dressings. However, these often fail in effectively addressing infections, as they struggle to both absorb exudates and maintain optimal local moisture. The system here presented was conceptualized with a three-layer design: the outer layer made of a fibrous polycaprolactone (PCL) film, to act as a barrier for preventing microorganisms and impurities from reaching the wound; the intermediate layer formed of a sodium alginate (SA) hydrogel loaded with ampicillin (Amp) for fighting infections; and the inner layer comprised of a fibrous film of PCL and polyethylene glycol (PEG) for facilitating cell recognition and preventing wound adhesion. Thermal evaluations, degradation, wettability and release behavior testing confirmed the system resistance overtime. The sandwich demonstrated the capability for absorbing exudates (≈70 %) and exhibited a controlled release of Amp for up to 24 h. Antimicrobial testing was performed against Staphylococcus aureus and Escherichia coli, as representatives of Gram-positive and Gram-negative bacteria: >99 % elimination of bacteria. Cell cytotoxicity assessments showed high cytocompatibility levels, confirming the safety of the proposed sandwich system. Adhesion assays confirmed the system ease of detaching without mechanical effort (0.37 N). Data established the efficiency of the sandwich-like system, suggesting promising applications in infected wound care.
摘要：
伤口中微生物定植和感染的发展是慢性的标志。管理和治疗这些伤口的流行方法涉及敷料。然而,这些通常无法有效解决感染，因为他们努力吸收分泌物并保持最佳的局部水分。这里介绍的系统采用三层设计进行了概念化：外层由纤维状聚己内酯（PCL）薄膜制成，作为防止微生物和杂质到达伤口的屏障;中间层由载有氨苄青霉素(Amp)的藻酸钠(SA)水凝胶形成,用于抵抗感染;内层由PCL和聚乙二醇(PEG)的纤维膜构成,用于促进细胞识别和防止伤口粘连。热评估,降解，润湿性和释放行为测试证实了系统的电阻随时间变化。夹心显示出吸收流出物的能力（≈70％），并表现出Amp的受控释放长达24小时。对金黄色葡萄球菌和大肠杆菌进行了抗菌测试，作为革兰氏阳性和革兰氏阴性细菌的代表：>99％的细菌消除。细胞毒性评估显示高细胞相容性水平，确认建议的三明治系统的安全性。粘附性测定证实了在没有机械力(0.37N)的情况下系统易于分离。数据确定了三明治样系统的效率，在感染伤口护理中提出了有希望的应用。
