Abstract：
Honey bees play a pivotal role in shaping ecosystems and sustaining human health as both pollinators and producers of health-promoting products. However, honey bee colony mortality is on the rise globally, driven by various factors, including parasites, pesticides, habitat loss, poor nutrition, and climate change. This has far-reaching consequences for the environment, economy, and human welfare. While efforts to address these issues are underway, the current progress in electron paramagnetic resonance (EPR) instrumentation affords using the immense potential of this magnetic resonance technique to study small samples such as honey bees. This paper presents the pioneering 2D in vivo EPR imaging experiment on a honey bee, revealing the ongoing redox-status of bees\' intestines. This way, by monitoring the spatio-temporal changes of the redox-active spin-probes\' EPR signal, it is possible to gain access to valuable information on the course of ongoing bees\' pathologies and the prospect of following-up on the efficiency of applied therapies. Employing a selection of diverse spin-probes could further reveal pH levels and oxygen concentrations in bee tissues, allowing a noninvasive assessment of bee physiology. This approach offers promising strategies for safeguarding pollinators and understanding their biology, fostering their well-being and ecological harmony.
摘要：
蜜蜂作为传粉者和促进健康产品的生产者，在塑造生态系统和维持人类健康方面发挥着关键作用。然而,蜜蜂群体死亡率在全球范围内呈上升趋势,在各种因素的推动下，包括寄生虫,杀虫剂,栖息地丧失,营养不良,和气候变化。这对环境产生了深远的影响，经济,和人类福利。虽然正在努力解决这些问题，电子顺磁共振（EPR）仪器的当前进展提供了利用这种磁共振技术的巨大潜力来研究小样品，例如蜜蜂。本文介绍了在蜜蜂上进行的开创性的2D体内EPR成像实验，揭示蜜蜂肠道正在进行的氧化还原状态。这边,通过监测氧化还原活性自旋探针EPR信号的时空变化，有可能获得有关正在进行的蜜蜂的病理过程的有价值的信息，以及对应用疗法的效率进行随访的前景。使用多种自旋探针可以进一步揭示蜜蜂组织中的pH水平和氧气浓度，允许对蜜蜂生理学进行非侵入性评估。这种方法为保护传粉者和了解其生物学提供了有希望的策略，促进他们的福祉和生态和谐。
