Abstract：
Bisphosphonates and myo-inositol trispyrophosphate (ITPP) are two classes of difficult-to-detect polar drugs that are prohibited under the rules of racing. ITPP is a drug capable of increasing the amount of oxygen in hypoxic tissues, and studies have shown that administration of ITPP increases the maximal exercise capacity in mice. The properties of ITPP make it an ideal candidate as a doping agent to enhance performance in racehorses. In recent years, ITPP had indeed been detected in racehorses and confiscated items. As for bisphosphonates, it is especially critical to control their use as since February 2019, the International Agreement on Breeding, Racing and Wagering (IABRW) by the International Federation of Horseracing Authorities (IFHA) had identified specific conditions on which bisphosphonates should not be administered to a racehorse. A recent review of literature shows that there is yet a simultaneous screening method for detecting ITPP and bisphosphonates in equine samples. This paper describes an efficient ion chromatography high-resolution mass spectrometry (IC-HRMS) method for the simultaneous detection of ITPP and 10 bisphosphonates at sub-parts-per-billion (ppb) to low-ppb levels in equine plasma after solid-phase extraction (SPE) and its application to an administration study of clodronic acid in horses.
摘要：
双膦酸盐和肌醇三磷酸（ITPP）是两类难以检测的极性药物，在竞赛规则下被禁止。ITPP是一种能够增加缺氧组织中氧气含量的药物，研究表明，服用ITPP可以增加小鼠的最大运动能力。ITPP的特性使其成为提高赛马性能的掺杂剂的理想候选物。近年来,ITPP确实在赛马和没收的物品中被发现。至于双膦酸盐，控制它们的使用尤为重要，因为自2019年2月以来，国际育种协议，国际赛马机构联合会（IFHA）的赛马和下注（IABRW）已经确定了不应向赛马施用双膦酸盐的具体条件。最近的文献综述显示,还存在用于检测马样品中的ITPP和双膦酸盐的同时筛选方法。本文介绍了一种有效的离子色谱高分辨率质谱（IC-HRMS）方法，该方法可同时检测马血浆中十亿分之一（ppb）至低ppb水平的ITPP和10种二膦酸盐固相萃取（SPE）及其在马中氯膦酸给药研究中的应用。
