Abstract：
Postoperative tumor recurrence and wound infection remain significant clinical challenges in surgery, often requiring adjuvant therapies. The combination treatment of photothermal therapy (PTT) and chemodynamic therapy (CDT) has proven to be effective in cancer treatment and wound infection. However, the hyperthermia during PTT increases the risk of normal tissue damage, severely impeding its application. Moreover, the efficacy of CDT is limited by insufficient hydrogen peroxide (H2O2) and excessive glutathione (GSH) levels at tumor or infection sites. Herein, an injectable and multifunctional CuO2@Au hydrogel system (CuO2@Au Gel) is developed for synergistic CDT and low-temperature PTT (LTPTT) to prevent tumor recurrence and bacterial wound infections. CuO2@Au Gel is constructed by embedding therapeutic CuO2@Au into low-melting point agarose hydrogel. In vitro and in vivo experiments confirm that the CuO2@Au in CuO2@Au Gel is capable of self-supplying H2O2 and depleting GSH, exhibiting effective CDT effect in acidic tumor or bacterial infected microenvironment. Additionally, it exhibits favorable photothermal conversion ability, inducing localized temperature elevation and synergistically enhancing CDT efficiency. The prepared CuO2@Au Gel demonstrates efficient tumor ablation capability in post-surgery recurrence mouse models and exhibits promising anti-infective efficiency in bacterial infection wound models, indicating significant potential in adjuvant therapy for post-surgical treatment and recovery.
摘要：
术后肿瘤复发和伤口感染仍然是外科手术中的重大临床挑战,经常需要辅助治疗。光热疗法（PTT）和化学动力学疗法（CDT）的组合治疗已被证明在癌症治疗和伤口感染中有效。然而,PTT期间的高温会增加正常组织损伤的风险，严重阻碍其应用。此外，CDT的疗效受到肿瘤或感染部位过氧化氢(H2O2)不足和谷胱甘肽(GSH)水平过高的限制.在这里，开发了一种可注射的多功能CuO2@Au水凝胶系统（CuO2@AuGel），用于协同CDT和低温PTT（LTPTT），以防止肿瘤复发和细菌性伤口感染。CuO2@Au凝胶是通过将治疗性CuO2@Au嵌入低熔点琼脂糖水凝胶中来构建的。体外和体内实验证实，CuO2@Au凝胶中的CuO2@Au能够自我供应H2O2并消耗GSH，在酸性肿瘤或细菌感染的微环境中表现出有效的CDT效应。此外,它表现出良好的光热转换能力，诱导局部温度升高，协同提高CDT效率。制备的CuO2@Au凝胶在术后复发小鼠模型中显示出有效的肿瘤消融能力，并在细菌感染伤口模型中显示出有希望的抗感染功效，表明在术后治疗和恢复的辅助治疗中具有重要的潜力。本文受版权保护。保留所有权利。
