PDF(Pubmed)
Abstract：
BACKGROUND: The intestine of young ruminants is in the developmental stage and has weaker resistance to the changes of external environment. Improving intestinal health is vital to promoting growth of young ruminants. This study investigated effects of guanidino acetic acid (GAA) and rumen-protected betaine (RPB) supplementation on growth, dietary nutrient digestion and GAA metabolism in the small intestine of sheep.
METHODS: Eighteen healthy Kazakh rams (27.46 ± 0.10 kg of body weight and 3-month old) were categorized into control, test group I and test group II, which were fed a basal diet, 1500 mg/kg GAA and 1500 mg/kg GAA + 600 mg/kg RPB, respectively.
RESULTS: Compared with control group, test group II had increased (p < 0.05) average daily gain, plasma creatine level, ether extract (EE) and phosphorus digestibility on day 30. On day 60, the EE apparent digestibility, jugular venous plasma GAA, GAA content in the duodenal mucosa and GAA content in the jejunal and ileal mucosa of test group II were higher (p < 0.05) than other groups. Transcriptome analysis revealed that the differentially expressed genes (DEGs) involved in the duodenal pathways of oxidative phosphorylation and non-alcoholic fatty liver disease were significantly altered in test group II versus test group I (p < 0.05). Moreover, in the jejunum, the MAPK signalling pathway, complement and coagulation cascade and B-cell receptor signalling pathway were significantly enriched, with ATPase, solute carrier transporter protein, DHFR, SI, GCK, ACACA and FASN being the significantly DEGs (p < 0.05).
CONCLUSIONS: Dietary supplementation of RPB on top of GAA in sheep diets may promote sheep growth and development by improving the body\'s energy, amino acid, glucose and lipid metabolism capacity.
METHODS: Eighteen healthy Kazakh rams (27.46 ± 0.10 kg of body weight and 3-month old) were categorized into control, test group I and test group II, which were fed a basal diet, 1500 mg/kg GAA and 1500 mg/kg GAA + 600 mg/kg RPB, respectively.
RESULTS: Compared with control group, test group II had increased (p < 0.05) average daily gain, plasma creatine level, ether extract (EE) and phosphorus digestibility on day 30. On day 60, the EE apparent digestibility, jugular venous plasma GAA, GAA content in the duodenal mucosa and GAA content in the jejunal and ileal mucosa of test group II were higher (p < 0.05) than other groups. Transcriptome analysis revealed that the differentially expressed genes (DEGs) involved in the duodenal pathways of oxidative phosphorylation and non-alcoholic fatty liver disease were significantly altered in test group II versus test group I (p < 0.05). Moreover, in the jejunum, the MAPK signalling pathway, complement and coagulation cascade and B-cell receptor signalling pathway were significantly enriched, with ATPase, solute carrier transporter protein, DHFR, SI, GCK, ACACA and FASN being the significantly DEGs (p < 0.05).
CONCLUSIONS: Dietary supplementation of RPB on top of GAA in sheep diets may promote sheep growth and development by improving the body\'s energy, amino acid, glucose and lipid metabolism capacity.
摘要：
背景：幼龄反刍动物的肠道处于发育阶段，对外部环境变化的抵抗力较弱。改善肠道健康对于促进幼龄反刍动物的生长至关重要。这项研究调查了胍基乙酸（GAA）和瘤胃保护甜菜碱（RPB）对生长的影响，饲粮营养消化和GAA在绵羊小肠中的代谢。
方法：将18只健康的哈萨克公羊（体重27.46±0.10kg，3个月大）归入对照组，试验组Ⅰ和试验组Ⅱ,以基础饮食喂养，1500mg/kgGAA和1500mg/kgGAA+600mg/kgRPB，分别。
结果：与对照组相比，试验组II平均日增重增加(p<0.05)，血浆肌酸水平,醚提取物(EE)和磷消化率在第30天。在第60天，EE表观消化率，颈静脉血浆GAA，试验Ⅱ组十二指肠粘膜GAA含量和空肠、回肠粘膜GAA含量均高于其他各组(p<0.05)。转录组分析显示，参与氧化磷酸化和非酒精性脂肪肝的十二指肠途径的差异表达基因（DEGs）在试验组II与试验组I中发生了显着变化（p<0.05）。此外，在空肠，MAPK信号通路,补体和凝血级联和B细胞受体信号通路显著富集，用ATPase,溶质载体转运蛋白，DHFR,SI,GCK,ACACA和FASN是显著的DEG(p<0.05)。
结论：在绵羊日粮中在GAA的基础上补充RPB可能通过提高身体能量来促进绵羊的生长发育。氨基酸,葡萄糖和脂质代谢能力。
方法：将18只健康的哈萨克公羊（体重27.46±0.10kg，3个月大）归入对照组，试验组Ⅰ和试验组Ⅱ,以基础饮食喂养，1500mg/kgGAA和1500mg/kgGAA+600mg/kgRPB，分别。
结果：与对照组相比，试验组II平均日增重增加(p<0.05)，血浆肌酸水平,醚提取物(EE)和磷消化率在第30天。在第60天，EE表观消化率，颈静脉血浆GAA，试验Ⅱ组十二指肠粘膜GAA含量和空肠、回肠粘膜GAA含量均高于其他各组(p<0.05)。转录组分析显示，参与氧化磷酸化和非酒精性脂肪肝的十二指肠途径的差异表达基因（DEGs）在试验组II与试验组I中发生了显着变化（p<0.05）。此外，在空肠，MAPK信号通路,补体和凝血级联和B细胞受体信号通路显著富集，用ATPase,溶质载体转运蛋白，DHFR,SI,GCK,ACACA和FASN是显著的DEG(p<0.05)。
结论：在绵羊日粮中在GAA的基础上补充RPB可能通过提高身体能量来促进绵羊的生长发育。氨基酸,葡萄糖和脂质代谢能力。
