Abstract：
Research on the markers of autoimmune response in multiple sclerosis (MS) is still of great importance. The aim of our study was the evaluation of plasma 20S constitutive proteasome, 20S immunoproteasome, and cathepsin S concentrations as potential biomarkers of a relapsing-remitting type of MS (RRMS). Surface plasmon resonance imaging (SPRI) biosensors were used for the evaluation of protein concentrations. Plasma 20S constitutive proteasome, 20S immunoproteasome, and cathepsin S concentrations were significantly higher in RRMS patients compared to the control group. All three parameters were characterized by excellent usefulness in differentiating MS patients from healthy individuals (AUC equal to or close to 1.000). The plasma concentration of analyzed parameters was not correlated with severity of disability in the course of RRMS (EDSS value), the number of years from the first MS symptoms, the number of years from MS diagnosis, or the number of relapses within the 24-month observational period. Our study has shown that plasma concentrations of 20S constitutive proteasome, 20S immunoproteasome, and cathepsin S have promising potential in differentiating RRMS patients from healthy individuals. All of the analyzed parameters were found to be independent of the time of MS relapse and the severity of neurological symptoms. Hence, their potential as highly sensitive and independent circulating markers of RRMS suggests a stronger association with immunological activity (inflammatory processes) than with the severity of the disease.
摘要：
多发性硬化症（MS）中自身免疫反应标志物的研究仍然非常重要。我们研究的目的是评估血浆20S组成型蛋白酶体，20S免疫蛋白酶体,和组织蛋白酶S浓度作为复发缓解型MS（RRMS）的潜在生物标志物。表面等离子体共振成像（SPRI）生物传感器用于评估蛋白质浓度。血浆20S组成型蛋白酶体，20S免疫蛋白酶体,与对照组相比，RRMS患者的组织蛋白酶S浓度明显更高。所有三个参数的特征在于在区分MS患者与健康个体(AUC等于或接近1.000)中的优异有用性。分析参数的血浆浓度与RRMS（EDSS值）过程中的残疾严重程度无关，从第一次出现MS症状开始的年数，MS诊断后的年数，或24个月观察期内复发的次数。我们的研究表明，20S组成型蛋白酶体的血浆浓度，20S免疫蛋白酶体,和组织蛋白酶S在区分RRMS患者与健康个体方面具有很好的潜力。发现所有分析的参数与MS复发的时间和神经系统症状的严重程度无关。因此，它们作为RRMS的高度敏感和独立的循环标志物的潜力表明与免疫活性(炎症过程)的关联比与疾病的严重程度更强.
