PDF(Pubmed)
Abstract：
BACKGROUND: Silica-sprayed tubes (SSTs) are often used to transport synovial fluid samples in equine practice. They promote the coagulation of the sample. The objective of the study is to evaluate the effect of SST on bacterial culture.
METHODS: The study was divided into two parts: sterile saline (Part A) and synovial fluid (Part B). Four common bacteria associated with equine synovial sepsis were used: Streptococcus pyogenes, Escherichia coli, Staphylococcus aureus and methicillin-resistant S. aureus (MRSA). Three collection tubes were used: STT, plain (no-additives) and brain and heart infusion (BHI) broth. Bacteria were cultured in horse blood agar plates for 48 h. Outcome variables were negative culture, positive culture and total number of colony-forming units (CFUs). Statistical analysis was performed using Mann-Whitney U test, and significance was set at p < 0.05.
RESULTS: The total number of agar plates read was 1557 (779 saline; 778 synovial fluid). Total negative cultures were 25/779 on saline and 3/778 on synovial fluid. In broth, maximum growth CFU was achieved after 8 h for both saline and synovial fluid for all bacteria. S. pyogenesand E. coli produced a significantly lower number of CFU when in SST compared to plain or broth after 4 h, whereas S. aureus (American Type Culture Collection [ATCC] and MRSA) only after 24 h.
CONCLUSIONS: Silica-containing tubes reduced bacterial proliferation, whereas the use of a BHI broth provided the highest bacterial load in the sample. The use of SST may have a negative effect on bacterial proliferation in samples obtained from clinical cases.
METHODS: The study was divided into two parts: sterile saline (Part A) and synovial fluid (Part B). Four common bacteria associated with equine synovial sepsis were used: Streptococcus pyogenes, Escherichia coli, Staphylococcus aureus and methicillin-resistant S. aureus (MRSA). Three collection tubes were used: STT, plain (no-additives) and brain and heart infusion (BHI) broth. Bacteria were cultured in horse blood agar plates for 48 h. Outcome variables were negative culture, positive culture and total number of colony-forming units (CFUs). Statistical analysis was performed using Mann-Whitney U test, and significance was set at p < 0.05.
RESULTS: The total number of agar plates read was 1557 (779 saline; 778 synovial fluid). Total negative cultures were 25/779 on saline and 3/778 on synovial fluid. In broth, maximum growth CFU was achieved after 8 h for both saline and synovial fluid for all bacteria. S. pyogenesand E. coli produced a significantly lower number of CFU when in SST compared to plain or broth after 4 h, whereas S. aureus (American Type Culture Collection [ATCC] and MRSA) only after 24 h.
CONCLUSIONS: Silica-containing tubes reduced bacterial proliferation, whereas the use of a BHI broth provided the highest bacterial load in the sample. The use of SST may have a negative effect on bacterial proliferation in samples obtained from clinical cases.
摘要：
背景：在马的实践中，通常使用二氧化硅喷雾管（SST）来运输滑液样品。它们促进样品的凝结。该研究的目的是评估SST对细菌培养的影响。
方法：研究分为两部分：无菌盐水（A部分）和滑液（B部分）。使用了与马滑膜败血症相关的四种常见细菌：化脓性链球菌，大肠杆菌,金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌(MRSA)。使用了三个收集管:STT,普通（无添加剂）和脑和心脏输液（BHI）肉汤。细菌在马血琼脂平板中培养48小时。结果变量为阴性培养物，阳性培养物和菌落形成单位总数(CFU)。使用Mann-WhitneyU检验进行统计分析，并且显著性设定为p<0.05。
结果:琼脂平板读数总数为1557(779生理盐水;778滑液)。总阴性培养物在盐水中为25/779，在滑液中为3/778。在肉汤中，所有细菌的生理盐水和滑液均在8小时后达到最大生长CFU。在SST中，产脓菌和大肠杆菌在4小时后与普通或肉汤相比，产生的CFU数量显着降低，而金黄色葡萄球菌(美国典型培养物保藏中心[ATCC]和MRSA)仅在24小时后。
结论：含二氧化硅管减少细菌增殖，而BHI肉汤的使用提供了样品中最高的细菌负荷。SST的使用可能对从临床病例获得的样品中的细菌增殖具有负面影响。
方法：研究分为两部分：无菌盐水（A部分）和滑液（B部分）。使用了与马滑膜败血症相关的四种常见细菌：化脓性链球菌，大肠杆菌,金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌(MRSA)。使用了三个收集管:STT,普通（无添加剂）和脑和心脏输液（BHI）肉汤。细菌在马血琼脂平板中培养48小时。结果变量为阴性培养物，阳性培养物和菌落形成单位总数(CFU)。使用Mann-WhitneyU检验进行统计分析，并且显著性设定为p<0.05。
结果:琼脂平板读数总数为1557(779生理盐水;778滑液)。总阴性培养物在盐水中为25/779，在滑液中为3/778。在肉汤中，所有细菌的生理盐水和滑液均在8小时后达到最大生长CFU。在SST中，产脓菌和大肠杆菌在4小时后与普通或肉汤相比，产生的CFU数量显着降低，而金黄色葡萄球菌(美国典型培养物保藏中心[ATCC]和MRSA)仅在24小时后。
结论：含二氧化硅管减少细菌增殖，而BHI肉汤的使用提供了样品中最高的细菌负荷。SST的使用可能对从临床病例获得的样品中的细菌增殖具有负面影响。
