OBJECTIVE: We investigated the correlation between Tr fluorescence and key wound healing parameters, including re-epithelialization, fibrosis, neovascularization, and acute and chronic inflammation, using a rabbit model.
METHODS: Seven rabbits underwent wound healing assessment over a 15-day period. We employed histological analysis from central and marginal biopsies, and UV fluorescence imaging captured by a monochromatic near-UV sensitive camera equipped with a passband optical filter (340 nm/12 nm). Excitation was achieved using a 295 nm LEDs ring lamp. Normalized fluorescence values were correlated with histological measurements using Pearson correlation.
RESULTS: The UV fluorescence strongly exhibited a strong correlation with re-epithelization (r = 0.8) at the wound edge, with peak intensity observed between the sixth and ninth days. Notably, wound-healing dynamics differed between the wound center and edge, primarily attributed to variations in re-epithelialization, neovascularization, and chronic inflammation.
CONCLUSIONS: Our findings highlight the presence of autofluorescence at 295/340 nm during wound healing, demonstrating a robust association with re-epithelialization. This excitation/emission signal holds promise as a valuable noninvasive strategy for monitoring wound closure, re-epithelialization, and other biological processes where Tr plays a pivotal role.
目的:我们研究了Tr荧光与关键伤口愈合参数之间的相关性,包括再上皮化,纤维化,新生血管形成,急性和慢性炎症,用兔子模型.
方法:七只兔子在15天的时间内接受了伤口愈合评估。我们采用了中央和边缘活检的组织学分析,和由配备有通带滤光器(340nm/12nm)的单色近UV敏感相机捕获的UV荧光成像。使用295nmLED环形灯实现激发。使用Pearson相关性将归一化的荧光值与组织学测量相关联。
结果:紫外线荧光与伤口边缘的再上皮化(r=0.8)强烈相关,在第六天和第九天之间观察到峰值强度。值得注意的是,伤口愈合动力学在伤口中心和边缘之间有所不同,主要归因于再上皮化的变化,新生血管形成,慢性炎症。
结论:我们的发现强调了伤口愈合过程中295/340nm处的自发荧光的存在,证明与上皮再形成有密切的联系。这种激发/发射信号有望成为监测伤口闭合的有价值的非侵入性策略。再上皮化,和其他生物过程,其中Tr起着关键作用。